'Candidatus anaplasma camelii' in onehumped camels (Camelus dromedarius) in Morocco: a novel and emerging anaplasma species?

Autor: Zaid Zouagui, Henri-Jean Boulouis, Marie J. Ducrotoy, Hicham Ait Lbacha, Abdelkbir Rhalem, Renaud Maillard, Elisabeth Petit, Said Alali
Přispěvatelé: Institut Agronomique et Vétérinaire Hassan II - IAV (MOROCCO) (IAV), Département de médecine, chirurgie et reproduction, Madinat Al Irfane, Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Division of Infection and Pathway Medicine, School of Biomedical Sciences, College of Medicine and Veterinary Medicine, University of Edinburgh, CEVA Santé Animale, Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, PRAD project [28027YM], Institut Agronomique et Veterinaire Hassan II, BIPAR, Ecole Nationale Veterinaire de Toulouse, Institut Agronomique et Vétérinaire Hassan II (IAV Hassan II), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Lbacha, Hicham Ait
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Infectious Diseases of Poverty
Infectious Diseases of Poverty, BMC, 2017, 6, pp.1-8. ⟨10.1186/s40249-016-0216-8⟩
Infectious Diseases of Poverty (6), 1-8. (2017)
ISSN: 2095-5162
2049-9957
Popis: Background There has been a growing interest in camel anaplasmosis due to its recent emergence in this reservoir species and concerns for its zoonotic potential. The epidemiology of anaplasmosis in camels therefore remains poorly understood mostly because camels belong to marginalised poor and often transhumant populations whose interests are largely neglected. Most studies of anaplasmosis in camels have relied on microscopy and serology for diagnosis and only three studies, undertaken in Tunisia, Saudia Arabia and China, have used molecular diagnostics. The present work characterises Anaplasmataceae strains circulating in the Camelus dromedarius reservoir in Morocco using PCR. Methods Camels (n = 106) were randomly sampled from 6 regions representing different agro-ecological areas in southern Morocco. Whole blood was collected and screened using PCR methods targeting the gene groEL. Anaplasmataceae strains were characterised by sequence analysis of the gene groEL. Results A total of 39.62% (42/106) camels screened were positive for Anaplasmataceae spp. GenBank BLAST analysis of five positive sequenced samples revealed that all strains were 100% identical to “Candidatus Anaplasma camelii”. Phylogenetic investigation and genetic characterisation of the aligned segment (650 bp) of the gene groEL confirmed high similarity with A. platys. Conclusion This study demonstrates the circulation of a previously unidentified species of the genus Anaplasma in Morocco which is genetically close to the agent causing canine anaplasmosis but whose main reservoir is thought to be Camelus dromedarius. Trial registration number This study is not a clinical trial and therefore a trial registration number does not apply. Electronic supplementary material The online version of this article (doi:10.1186/s40249-016-0216-8) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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