Macrophage migration inhibitory factor promoter polymorphisms (−794 CATT5–8): Relationship with soluble MIF levels in coronary atherosclerotic disease subjects

Autor: Shaoze Wu, Lu-ping Wang, Jifei Tang, Lu Qian, Xiaoyan Wang, Jie Wang, Jiaoni Wang, Ji Li, Gao-Jiang Luo, Luyuan Tao, Saroj Thapa, Kangting Ji
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
medicine.medical_specialty
lcsh:Diseases of the circulatory (Cardiovascular) system
Enzyme-Linked Immunosorbent Assay
Coronary Artery Disease
Macrophage Migration Inhibitory Factor
Coronary Angiography
Polymerase Chain Reaction
Risk Assessment
Severity of Illness Index
Coronary artery disease
03 medical and health sciences
0302 clinical medicine
Gene Frequency
Predictive Value of Tests
Risk Factors
Genotype
Coronary Atherosclerotic Disease
Gensini’s degree integral
medicine
otorhinolaryngologic diseases
Humans
Genetic Predisposition to Disease
Promoter Regions
Genetic
Allele frequency
Genotyping
Macrophage Migration-Inhibitory Factors
Genetic Association Studies
Angiology
Retrospective Studies
Polymorphism
Genetic
business.industry
Coronary Stenosis
Gene polymorphisms
medicine.disease
Genotype frequency
Intramolecular Oxidoreductases
030104 developmental biology
Phenotype
lcsh:RC666-701
030220 oncology & carcinogenesis
Predictive value of tests
Immunology
Macrophage migration inhibitory factor
Cardiology and Cardiovascular Medicine
business
Research Article
Zdroj: BMC Cardiovascular Disorders, Vol 17, Iss 1, Pp 1-5 (2017)
BMC Cardiovascular Disorders
ISSN: 1471-2261
DOI: 10.1186/s12872-017-0570-x
Popis: Background We analyzed the relationship of −794 CATT5–8 MIF polymorphisms with soluble MIF in Coronary Atherosclerotic Disease (CAD) patients. Methods A total of 256 patients selected, on which 186 normal-coronary and 70 Coronary artery disease subjects, were recruited in the study (Retrospectively registered). Genotyping of −794 CATT5–8 polymorphisms were performed by PCR and DNA sequencing. Serum MIF levels were measured using an ELISA kit. Patients were classified by coronary angiogram, and CAD based on Gensini’s integral degree (angiographic scoring system). Results The allele frequency and genotype frequency of −794 CATT5–8 did not show any differences in normal-coronary subjects and CAD subjects. In CAD patients, serum MIF levels was lower in CATT (5) subjects than in CATT (7) subjects, while the genotype of −794 CATT5–8 did not show differences in serum MIF levels. In addition, we found a decrease in serum MIF levels in carriers of the (5/5) genotypes the −794 CATT5–8 MIF polymorphisms, although it was not significant. There was no relationship of CAD class and the allele frequency of −794 CATT5–8. Conclusions This study found no association between CAD class and −794 CATT5–8 MIF polymorphisms with soluble MIF levels in CAD Subjects. Trial registration NCT01750502 (November 2012, Retrospectively registered).
Databáze: OpenAIRE
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