Minimal cross-recombination between wild-type and loxP511 sites in vivo facilitates truncating both ends of large DNA inserts in pBACe3.6 and related vectors
| Autor: | Douglas M. Garland, Ken R. Harewood, Deepak Srivastava, Pradeep K. Chatterjee, Leighcraft A. Shakes |
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| Rok vydání: | 2005 |
| Předmět: |
Transposable element
Chromosomes Artificial Bacterial Mutant Cre recombinase Biology Regulatory Sequences Nucleic Acid Insert (molecular biology) 03 medical and health sciences chemistry.chemical_compound Viral Proteins Plasmid Transduction Genetic Genetics Bacteriophage P1 Deoxyribonucleases Type II Site-Specific Gene 030304 developmental biology DNA Primers Sequence Deletion Recombination Genetic 0303 health sciences Base Sequence Integrases 030302 biochemistry & molecular biology DNA Mutagenesis Insertional chemistry Regulatory sequence DNA Transposable Elements Methods Online Plasmids |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 |
| Popis: | Contrary to several earlier reports, we find that cross-recombination between wild-type and the mutant loxP511 sites is |
| Databáze: | OpenAIRE |
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