Cardiosphere-derived cells, with and without a biological scaffold, stimulate myogenesis and recovery of muscle function in mice with volumetric muscle loss
| Autor: | Lizbeth Sanchez, Yena Lee, Alessandra Ciullo, Jocelyn Alfaro, Liang Li, Alice Rannou, Mario Fournier, Russell G. Rogers, Eduardo Marbán, Weixin Liu, Kiel Peck |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Cell
Biophysics Bioengineering 02 engineering and technology Muscle Development Biomaterials Extracellular matrix Mice 03 medical and health sciences Muscular Diseases Extracellular Animals Humans Regeneration Medicine Myocyte Muscle Skeletal 030304 developmental biology 0303 health sciences business.industry Myogenesis Skeletal muscle 021001 nanoscience & nanotechnology Cell biology Transplantation Gene expression profiling medicine.anatomical_structure Mechanics of Materials Mice Inbred mdx Ceramics and Composites 0210 nano-technology business |
| Zdroj: | Biomaterials. 274:120852 |
| ISSN: | 0142-9612 |
| Popis: | Extremity trauma to military personnel and civilians commonly results in volumetric muscle loss (VML), leaving patients suffering chronic physical disability. Biomaterial-based technologies such as extracellular matrices (ECMs) are currently in clinical testing for soft tissue repair, but, in preclinical models of VML, the efficacy of ECMs is equivocal. In a murine model of VML, we investigated the effects of ECM and/or cardiosphere-derived cell (CDC) therapy; the latter improves skeletal myogenesis and muscle function in mdx mice, so we reasoned that CDCs may exert disease-modifying bioactivity in VML. While ECM alone improves functional recovery, CDCs have no additive or synergistic benefits with ECM transplantation following VML injury. However, CDCs alone are sufficient to promote muscle recovery, leading to sustained increases in muscle function throughout the study period. Notably, CDCs stimulate satellite cell accumulation in the muscle defect area and hasten myogenic progression (as evidenced by qPCR gene expression profiling), leading to global increases in myofiber numbers and anterior muscle compartment volume. Together, these data implicate CDCs as a viable therapeutic candidate to regenerate skeletal muscle injured by VML. |
| Databáze: | OpenAIRE |
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