Neuromodulators of the lingual von Ebner gland: an immunocytochemical study
| Autor: | S. E. Solomon, O. A. Brusco, I. M. Roberts, J. J. Bernstein, W. Goldberg |
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| Rok vydání: | 1991 |
| Předmět: |
Male
Serotonin medicine.medical_specialty Pathology Histology Tyrosine 3-Monooxygenase Vasoactive intestinal peptide Immunocytochemistry Substance P Biology Choline O-Acetyltransferase Immunoenzyme Techniques chemistry.chemical_compound Tongue stomatognathic system Internal medicine medicine Animals Molecular Biology Neurotransmitter Agents Rats Inbred Strains Von Ebner Glands Lipase Cell Biology General Medicine Choline acetyltransferase Rats Medical Laboratory Technology Serous fluid medicine.anatomical_structure Endocrinology chemistry biology.protein Anatomy Somatostatin General Agricultural and Biological Sciences Lingual lipase |
| Zdroj: | Histochemistry. 96:153-156 |
| ISSN: | 1432-119X 0301-5564 |
| Popis: | The serous lingual glands of von Ebner secrete lingual lipase, an enzyme that begins fat digestion in the stomach. The objective of this study was to characterize the neuromodulators in the rat tongue and von Ebner glands using immunocytochemical techniques. Rat lingual tissues were fixed in formalin, embedded in paraffin and sectioned at 4 microns for light microscopic studies. Immunocytochemical localization of neuromodulators was performed with monospecific anti-rat neuromodulator IgG or control (preimmune) IgG as the primary antibody, using the peroxidase-antiperoxidase (PAP) technique. No staining was seen with control anti-rat IgG. Immunospecific staining for vasoactive intestinal peptide (VIP), tyrosine hydroxylase and choline acetyltransferase (CHAT) was observed in nerves in the tongue, and cells containing immunospecific staining for serotonin (5-hydroxytryptamine) were seen in the stroma between the lingual glands. Selected cells in the serous glands stained positively for the presence of substance P and somatostatin. Adrenergic, VIP-containing and cholinergic nerves appear to innervate the tongue and serous glands. Substance P and somatostatin were identified in cells of the lingual serous glands and may be additional local modulators regulating lingual lipase release. |
| Databáze: | OpenAIRE |
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