Cultivation and functional characterization of 79 planctomycetes uncovers their unique biology
| Autor: | Alfred M. Spormann, Olga Jeske, Marnix H. Medema, Thorsten Mascher, Stijn H Peeters, Franz Brümmer, Mike S. M. Jetten, Peter Hornburger, Manfred Rohde, Christian Jogler, Michael Y. Galperin, Jörg Overmann, Mareike Jogler, Ralph Walter Müller, Christian Boedeker, Rudolf Amann, Anja Heuer, Daniela Pinto, Matthias Labrenz, Elena Rivas-Marín, Patrick Rast, Damien P. Devos, Sonja Oberbeckmann, Olga Maria Lage, Broder J. Merkel, Thomas Pohl, Sandra Wiegand, Timo Kohn, Anke Meyerdierks, Lise Øvreås, John Vollmers, Julia E. Storesund, Anne-Kristin Kaster, Sebastian Lücker, Boyke Bunk, Nicolai Kallscheuer, Huub J. M. Op den Camp |
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| Přispěvatelé: | German Research Foundation, Volkswagen Foundation, National Institutes of Health (US), Medical Library Association, Federal Ministry of Science, Research and Economy (Germany), Max Planck Society, Centro Interdisciplinar de Investigação Marinha e Ambiental |
| Rok vydání: | 2020 |
| Předmět: |
cell division
ved/biology.organism_classification_rank.species Secondary Metabolism genetic analysis phylogeny Applied Microbiology and Biotechnology taxonomy Laboratory of Geo-information Science and Remote Sensing bacterial genome RNA Ribosomal 16S genetics Clade Phylogeny cladistics 0303 health sciences biology bacterial structures species habitat Planctomycetes Bacterial Physiological Phenomena bacterium 3. Good health priority journal classification bacterium identification aquatic environment Cell Division signal transduction Signal Transduction Microbiology (medical) amino acid analysis RNA 16S Bioinformatics Immunology bacterium culture Bacterial genome size gene sequence Microbiology DNA sequencing Article 03 medical and health sciences Phylogenetics Bioinformatica Genetics Life Science Laboratorium voor Geo-informatiekunde en Remote Sensing 14. Life underwater Model organism bacterial phenomena and functions Ecosystem 030304 developmental biology Ecological niche growth development and aging nonhuman electron microscopy Bacteria 030306 microbiology ved/biology Genetic Variation Cell Biology biology.organism_classification bacterial strain bacterial cell Evolutionary biology Ecological Microbiology microbial diversity cytology EPS Genome Bacterial cell structure |
| Zdroj: | Nature Microbiology, 5, 126-140 Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Nature Microbiology, 5, 1, pp. 126-140 Nature Microbiology Digital.CSIC. Repositorio Institucional del CSIC instname Nature microbiology United States England Nat Microbiol Nature Microbiology 5 (2019) |
| ISSN: | 2058-5276 |
| Popis: | When it comes to the discovery and analysis of yet uncharted bacterial traits, pure cultures are essential as only these allow detailed morphological and physiological characterization as well as genetic manipulation. However, microbiologists are struggling to isolate and maintain the majority of bacterial strains, as mimicking their native environmental niches adequately can be a challenging task. Here, we report the diversity-driven cultivation, characterization and genome sequencing of 79 bacterial strains from all major taxonomic clades of the conspicuous bacterial phylum Planctomycetes. The samples were derived from different aquatic environments but close relatives could be isolated from geographically distinct regions and structurally diverse habitats, implying that ‘everything is everywhere’. With the discovery of lateral budding in ‘Kolteria novifilia’ and the capability of the members of the Saltatorellus clade to divide by binary fission as well as budding, we identified previously unknown modes of bacterial cell division. Alongside unobserved aspects of cell signalling and small-molecule production, our findings demonstrate that exploration beyond the well-established model organisms has the potential to increase our knowledge of bacterial diversity. We illustrate how ‘microbial dark matter’ can be accessed by cultivation techniques, expanding the organismic background for small-molecule research and drug-target detection. This work was funded by the Deutsche Forschungsgemeinschaft (grant no. JO 893/4-1) and the Volkswagen foundation (experiment no. 89256). M.Y.G. was funded by the NIH IRP at the US National Library of Medicine. Work in the Mascher lab was supported by the Deutsche Forschungsgemeinschaft (grant no. MA2837/2-2) and the Bundeministerium für Bildung und Forschung in the framework of the ERAnet Synthetic Biology (project: ERASynBio2-ECFexpress). R.A. and A.M. were funded by the Max Planck Society. |
| Databáze: | OpenAIRE |
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