Synthetic microbial consortium with specific roles designated by genetic circuits for cooperative chemical production
| Autor: | Taizo Hanai, Kenshiro Iwasaki, Hiroyuki Hamada, Hiroshi Honjo, Keigo Tsuruno, Yuki Soma |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Cellobiose Microorganism Microbial Consortia Bioengineering medicine.disease_cause 01 natural sciences Applied Microbiology and Biotechnology 2-Propanol 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology medicine Escherichia coli Gene 030304 developmental biology chemistry.chemical_classification 0303 health sciences Chemistry Strain (biology) food and beverages Microbial consortium Metabolic pathway Enzyme Glucose Biochemistry Microorganisms Genetically-Modified Biotechnology |
| Zdroj: | Metabolic engineering. 55 |
| ISSN: | 1096-7184 |
| Popis: | Synthetic microbial consortia consisting of microorganisms with different synthetic genetic circuits or divided synthetic metabolic pathway components can exert functions that are beyond the capacities of single microorganisms. However, few consortia of microorganisms with different synthetic genetic circuits have been developed. We designed and constructed a synthetic microbial consortium composed of an enzyme-producing strain and a target chemical-producing strain using Escherichia coli for chemical production with efficient saccharification. The enzyme-producing strain harbored a synthetic genetic circuit to produce beta-glucosidase, which converts cellobiose to glucose, destroys itself via the lytic genes, and release the enzyme when the desired cell density is reached. The target chemical-producing strain was programmed by a synthetic genetic circuit to express enzymes in the synthetic metabolic pathway for isopropanol production when the enzyme-producing strain grows until release of the enzyme. Our results demonstrate the benefits of synthetic microbial consortia with distributed tasks for effective chemical production from biomass. |
| Databáze: | OpenAIRE |
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