ABC transportome inventory of human pathogenic yeast Candida glabrata: Phylogenetic and expression analysis
Autor: | Mohit Kumar, Priya Kumari, Poonam Vishwakarma, Andrew M. Lynn, Naseem A. Gaur, Sonam Kumari, Rajendra Prasad, Suman Sharma, Nitesh Kumar Khandelwal, Garima Shahi, Mahendra Varma |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
030106 microbiology Protein domain lcsh:Medicine ATP-binding cassette transporter Candida glabrata Genome 03 medical and health sciences Protein Domains Phylogenetics Humans lcsh:Science Gene Phylogeny Genetics Multidisciplinary biology Gene Expression Profiling lcsh:R Fungal genetics biology.organism_classification 030104 developmental biology Membrane protein lcsh:Q ATP-Binding Cassette Transporters Chromosomes Fungal |
Zdroj: | PLoS ONE, Vol 13, Iss 8, p e0202993 (2018) |
ISSN: | 1932-6203 |
Popis: | ATP-binding cassette (ABC) is one of the two major superfamilies of transporters present across the evolutionary scale. ABC superfamily members came to prominence due to their ability to extrude broad spectrum of substrates and to confer multi drug resistance (MDR). Overexpression of some ABC transporters in clinical isolates of Candida species was attributed to the development of MDR phenotypes. Among Candida species, Candida glabrata is an emerging drug resistant species in human fungal infections. A comprehensive analysis of such proteins in C. glabrata is required to untangle their role not only in MDR but also in other biological processes. Bioinformatic analysis of proteins encoded by genome of human pathogenic yeast C. glabrata identified 25 putative ABC protein coding genes. On the basis of phylogenetic analysis, domain organization and nomenclature adopted by the Human Genome Organization (HUGO) scheme, these proteins were categorized into six subfamilies such as Pleiotropic Drug Resistance (PDR)/ABCG, Multi Drug Resistance (MDR)/ABCB, Multi Drug Resistance associated Protein (MRP)/ABCC, Adrenoleukodystrophy protein (ALDp)/ABCD, RNase L Inhibitor (RLI)/ABCE and Elongation Factor 3 (EF3)/ABCF. Among these, only 18 ABC proteins contained transmembrane domains (TMDs) and were grouped as membrane proteins, predominantly belonging to PDR, MDR, MRP, and ALDp subfamilies. A comparative phylogenetic analysis of these ABC proteins with other yeast species revealed their orthologous relationship and pointed towards their conserved functions. Quantitative real time PCR (qRT-PCR) analysis of putative membrane localized ABC protein encoding genes of C. glabrata confirmed their basal expression and showed variable transcriptional response towards antimycotic drugs. This study presents first comprehensive overview of ABC superfamily proteins of a human fungal pathogen C. glabrata, which is expected to provide an important platform for in depth analysis of their physiological relevance in cellular processes and drug resistance. |
Databáze: | OpenAIRE |
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