Memory T cells established by seasonal human influenza A infection cross-react with avian influenza A (H5N1) in healthy individuals
| Autor: | Yanchun Peng, Jeremy Farrar, Tao Dong, de Jong, Sarah Rowland-Jones, Lee Ly-H., Geoffrey L. Smith, Cameron P. Simmons, B A Askonas, Andrew J. McMichael, Chau Nvv., Townsend Arm., Ha Dla., R Schumacher |
|---|---|
| Přispěvatelé: | Medical Microbiology and Infection Prevention |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
CD4-Positive T-Lymphocytes
Proteome viruses T-Lymphocytes Epitopes T-Lymphocyte CD8-Positive T-Lymphocytes medicine.disease_cause Epitope Mice Clinical investigation Influenza A virus Medicine virus diseases General Medicine Middle Aged medicine.anatomical_structure Vietnam Health Influenza Vaccines Healthy individuals Seasons Research Article Adult Human influenza Vaccinia virus Biology Cross Reactions H5N1 genetic structure Antigenic drift Viral Matrix Proteins Influenza Human Animals Humans Heterosubtypic immunity Influenza A Virus H5N1 Subtype business.industry Influenza A Virus H3N2 Subtype Virology United Kingdom Influenza A virus subtype H5N1 Clarification Nucleoproteins Vaccines Inactivated Commentary business Memory T cell Immunologic Memory CD8 T-Lymphocytes Cytotoxic |
| Zdroj: | Journal of clinical investigation, 118(10), 3478-3490. The American Society for Clinical Investigation |
| ISSN: | 0021-9738 |
| Popis: | The threat of avian influenza A (H5N1) infection in humans remains a global health concern. Current influenza vaccines stimulate antibody responses against the surface glycoproteins but are ineffective against strains that have undergone significant antigenic variation. An alternative approach is to stimulate pre-existing memory T cells established by seasonal human influenza A infection that could cross-react with H5N1 by targeting highly conserved internal proteins. To determine how common cross-reactive T cells are, we performed a comprehensive ex vivo analysis of cross-reactive CD4+ and CD8+ memory T cell responses to overlapping peptides spanning the full proteome of influenza A/Viet Nam/CL26/2005 (H5N1) and influenza A/New York/232/2004 (H3N2) in healthy individuals from the United Kingdom and Viet Nam. Memory CD4+ and CD8+ T cells isolated from the majority of participants exhibited human influenza-specific responses and showed cross-recognition of at least one H5N1 internal protein. Participant CD4+ and CD8+ T cells recognized multiple synthesized influenza peptides, including peptides from the H5N1 strain. Matrix protein 1 (M1) and nucleoprotein (NP) were the immunodominant targets of cross-recognition. In addition, cross-reactive CD4+ and CD8+ T cells recognized target cells infected with recombinant vaccinia viruses expressing either H5N1 M1 or NP. Thus, vaccine formulas inducing heterosubtypic T cell-mediated immunity may confer broad protection against avian and human influenza A viruses. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|