Quantitative high performance liquid chromatographic analysis of diterpenoids in agni-casti fructus
| Autor: | Otto Sticher, Eva Hoberg, Beat H. Meier |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Pharmacology
p-Cymene Chromatography biology Chemistry Vitex Organic Chemistry Pharmaceutical Science Reproducibility of Results Plants biology.organism_classification High-performance liquid chromatography Agni casti fructus Vitex agnus-castus Analytical Chemistry chemistry.chemical_compound Complementary and alternative medicine Drug Discovery Molecular Medicine Rotundifuran Diterpenes Beta (finance) Medicinal plants Chromatography High Pressure Liquid |
| Zdroj: | Planta medica. 66(4) |
| ISSN: | 0032-0943 |
| Popis: | Pharmacological data have indicated that part of the dopaminergic activity of Vitex agnus-castus is attributed to the labdan diterpenoids found in the fruits. Therefore an analytical method for the standardization of rotundifuran (1), vitexilactone (2) and 6 beta,7 beta-diacetoxy-13-hydroxy-labda-8,14-diene (3) was developed. Because of the time-consuming and expensive isolation of the diterpenoids, p-cymene was chosen as an internal standard. The concentration of rotundifuran in different extracts and trade samples of the drug varies between 0.04 and 0.30% in the drug and between 1.04 and 2.23% in the extract. The concentration of vitexilactone was generally lower between 0.016 and 0.167% for the drug and between 0.34 and 1.01% for the extract. The determined concentration of 6 beta,7 beta-diacetoxy-13-hydroxy-labda-8,14-diene in the drug was in the range of 0.02 and 0.10% and in the extract in the range of 0.18 and 0.80%. Determination of the factors of correction of p-cymene gave 5.63 for rotundifuran, 2.73 for vitexilactone and 3.74 for 6 beta,7 beta-diacetoxy-13-hydroxy-labda-8,14-diene. |
| Databáze: | OpenAIRE |
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