Cellular miR-130b inhibits replication of porcine reproductive and respiratory syndrome virus in vitro and in vivo
| Autor: | Yifeng Jiang, Liwei Li, Lingxue Yu, Hao Zheng, Fei Gao, Yan-Jun Zhou, Guangzhi Tong, Tianqi Xia, Zehui Qu, Shen Yang, Wu Tong |
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| Rok vydání: | 2015 |
| Předmět: |
Untranslated region
viruses animal diseases Molecular Sequence Data Sus scrofa Virus Replication Antiviral Agents Article In vivo microRNA Animals Porcine respiratory and reproductive syndrome virus RNA Messenger Administration Intranasal Regulation of gene expression Multidisciplinary Genome biology Base Sequence Tumor Necrosis Factor-alpha RNA virus diseases Interferon-alpha respiratory system Porcine reproductive and respiratory syndrome virus biology.organism_classification Virology MicroRNAs Viral replication Gene Expression Regulation Tumor necrosis factor alpha 5' Untranslated Regions |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | MicroRNAs (miRNAs) can impact viral infections by binding to sequences with partial complementarity on viral RNA transcripts, usually resulting in the repression of virus replication. In the present study, we identified a potential binding site for miR-130 in the 5′ untranslated region (bps 155-162) of the porcine reproductive and respiratory syndrome virus (PRRSV) genome. We found that the delivery of multiple miR-130 family mimics, especially miR-130b, resulted in inhibition of PRRSV replication in vitro. miR-130 was effective in inhibiting the replication of multiple type 2 PRRSV strains, but not against vSHE, a classical type 1 strain. miR-130 over-expression did not induce IFN-α or TNF-α expression in either uninfected or PRRSV-infected porcine alveolar macrophages. Results from luciferase reporter assays indicated that miR-130 directly targeted the PRRSV 5′ UTR. Intranasal inoculation of piglets with miR-130b exhibited antiviral activity in vivo and partially protected piglets from an otherwise lethal challenge with HP-PRRSV strain vJX143. Overall, these results demonstrate the importance of the miR-130 family in modulating PRRSV replication and also provide a scientific basis for using cellular miRNAs in anti-PRRSV therapies. |
| Databáze: | OpenAIRE |
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