1,25-Dihydroxyvitamin D increases the gene expression of enzymes protecting from glucolipotoxicity in peripheral blood mononuclear cells and human primary endothelial cells
| Autor: | Lukáš Pácal, Katerina Kankova, Katarína Kuricová, Anna Pleskačová |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty medicine.medical_treatment 030209 endocrinology & metabolism Peripheral blood mononuclear cell vitamin D deficiency 03 medical and health sciences 0302 clinical medicine Immune system Internal medicine Insulin Secretion Human Umbilical Vein Endothelial Cells medicine Vitamin D and neurology Homeostasis Humans Insulin Vitamin D Cells Cultured 2. Zero hunger Regulation of gene expression Thiamin Pyrophosphokinase business.industry Lactoylglutathione Lyase General Medicine medicine.disease 3. Good health 030104 developmental biology Endocrinology Gene Expression Regulation Leukocytes Mononuclear Transketolase Beta cell business Food Science |
| Zdroj: | Food & Function. 7:2537-2543 |
| ISSN: | 2042-650X 2042-6496 |
| Popis: | Besides its classical function as an orchestrator of calcium and phosphorus homeostasis, vitamin D also affects insulin secretion and tissue efficiency. A number of studies have consistently reported the inverse relationship between vitamin D deficiency and type 2 diabetes. Activation of certain metabolic pathways and down-stream transcription factors may protect from glucolipotoxicity and their targeted activation – e.g. by vitamin D – might explain the detrimental role of vitamin D deficiency in diabetes. The aim of the study was to quantify gene and protein expression of selected enzymes involved in the protection from glucolipotoxicity, specifically glyoxalase 1 (GLO1), and other enzymes with antioxidant activity – hemoxygenase (HMOX), thiamin pyrophosphokinase (TPK1) and transketolase (TKT), under normo- and hyperglycemic conditions and upon addition of vitamin D in peripheral blood mononuclear cells (PBMCs) and human umbilical vein endothelial cells (HUVEC). The results of our study indicate that the active form of vitamin D regulates gene expression of enzymes opposing the harmful effect of glucolipotoxicity whose activities appear to be suppressed by hyperglycemia. However, we were unable to confirm this effect on protein expression. While we cannot speculate on the effect of vitamin D on diabetes itself our results support its role in the protection against existing glucolipotoxicity therefore possibly translating into the prevention of development of diabetic complications. |
| Databáze: | OpenAIRE |
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