Effect of heat shock on murine norovirus replication in RAW264.7 cells
| Autor: | Chang Ma, Jinwei You, Shifeng Yun, Jie Liu, Min Dong, Xuliang Zhang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
biology ved/biology medicine.medical_treatment 030106 microbiology ved/biology.organism_classification_rank.species medicine.disease_cause Microbiology Hsp90 Virology Hsp70 03 medical and health sciences 030104 developmental biology Infectious Diseases Cytokine Heat shock protein biology.protein Norovirus medicine Viral genome replication Pathogen Murine norovirus |
| Zdroj: | Microbial pathogenesis. 142 |
| ISSN: | 1096-1208 |
| Popis: | Murine norovirus (MNV), is a prevalent pathogen of laboratory mice closely related to human norovirus (HuNoV), a contagious pathogen known to cause gastroenteritis worldwide; however, the mechanism of norovirus replication remains poorly understood. Both heat shock protein 90 (Hsp90) and heat shock protein 70 (Hsp70) play an important role in viral genome replication and viral gene expression. In this study, we first found that heat stress exerted a positive effect on the replication of MNV in the murine macrophage RAW264.7 cell line. Inhibition of Hsp70 and Hsp90 by the specific inhibitors, KNK437 and 17-AGG, respectively showed that Hsp70 and Hsp90 enhanced MNV genome replication and virion production. In addition, we found that KNK437 and 17-AGG could decrease the level of IL-1β, IL-10, and TNF-α mRNA expression in MNV-infected cells. These data suggested that heat stress can positively regulate MNV replication, which advances our understanding of the molecular mechanism of MNV infection. |
| Databáze: | OpenAIRE |
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