Recombinant interferon-alpha selectively inhibits the production of interleukin-5 by human CD4+ T cells
| Autor: | Michel Goldman, A. Crusiaux, Elie Cogan, Liliane Schandené, G Del Prete, B. Kennes, Patrick Stordeur, Sergio Romagnani |
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| Rok vydání: | 1996 |
| Předmět: |
CD4-Positive T-Lymphocytes
CD3 Molecular Sequence Data Alpha interferon Gene Expression Platelet Glycoprotein GPIIb-IIIa Complex Interferon alpha-2 Lymphocyte Activation Transfection Interleukin 21 Mice Th2 Cells Downregulation and upregulation CD28 Antigens Hypereosinophilic Syndrome Animals Humans RNA Messenger Interleukin 5 Interleukin 4 DNA Primers biology Base Sequence Interferon-alpha General Medicine Molecular biology Recombinant Proteins Interleukin-10 Interleukin 10 biology.protein Interleukin-4 Interleukin-5 Research Article |
| Zdroj: | The Journal of clinical investigation. 97(2) |
| ISSN: | 0021-9738 |
| Popis: | The effects of recombinant IFN-alpha on the production of IL-5 by human CD4+ T cells were first analyzed on resting CD4+ T cells purified from normal PBMC and stimulated either with a combination of PMA and anti-CD28 mAb or anti-CD3 mAb cross-linked on B7-1/CD32-transfected mouse fibroblasts. We found that IFN-alpha profoundly inhibited in a dose-dependent manner IL-5 production by resting CD4+ T cells whereas IL-10 was upregulated in both systems. The addition of a neutralizing anti-IL-10 mAb to PMA and anti-CD28 mAb upregulated IL-5 production by resting CD4+ T cells but did not prevent IFN-alpha-induced IL-5 inhibition. We then analyzed the effect of IFN-alpha on the production of cytokines by differentiated type 2 helper (Th2) CD4+CD3- cells isolated from peripheral blood of two patients with the hypereosinophilic syndrome. In both cases, IFN-alpha markedly inhibited IL-5 production while it induced mild upregulation of IL-4 and IL-10. Finally, the inhibitory effect of IFN-alpha on IL-5 production was confirmed on a panel of Th2 and Th0 clones generated in vitro. In 2 out of 6 clones, IL-5 inhibition was associated with upregulation of IL-4 and IL-10. We conclude that IFN-alpha selectively downregulates IL-5 synthesis by human CD4+ T cells. |
| Databáze: | OpenAIRE |
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