Molecular characterization of a G protein α-subunit-encoding gene fromMucor circinelloides
| Autor: | Mario Pedraza-Reyes, Roberto Coria, Georgina E. Reyna-López, Guadalupe Martínez-Cadena, Laura Ongay-Larios, Jesús García-Soto, Víctor Meza-Carmen, José Arnau |
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| Rok vydání: | 2006 |
| Předmět: |
DNA
Complementary Sequence analysis Protein subunit Molecular Sequence Data Immunology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Fungal Proteins Complementary DNA Escherichia coli Genetics medicine Northern blot Molecular Biology Gene Phylogeny Sequence Homology Amino Acid Molecular mass biology General Medicine biology.organism_classification Molecular biology GTP-Binding Protein alpha Subunits Recombinant Proteins Biochemistry Mucor Mucor circinelloides Electrophoresis Polyacrylamide Gel |
| Zdroj: | Canadian Journal of Microbiology. 52:627-635 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/w06-010 |
| Popis: | Genes encoding the Gα subunit were cloned from Mucor circinelloides, a zygomycete dimorphic fungus. There are at least four genes that encode for Gα subunits, gpa1, gpa2, gpa3, and gpa4. The genes gpa1 and gpa3 were isolated and characterized, and their predicted products showed 36%–67% identity with Gα subunits from diverse fungi. Northern blot analysis of gpa3 showed that it is present in spores and constitutively expressed during mycelium development and during yeast–mycelium and mycelium–yeast transitions. However, during yeast cell growth, decreased levels of mRNA were observed. Sequence analysis of gpa3 cDNA revealed that Gpa3 encodes a polypeptide of 356 amino acids with a calculated molecular mass of 40.8 kDa. The deduced sequence of Gpa3 protein contains all the consensus regions of Gα subunits of the Gαi/o/tsubfamily except the cysteine near the C terminus for potential ADP-ribosylation by pertussis toxin. This cDNA was expressed in Escherichia coli and purified by affinity chromatography. Based on its electrophoretic mobility in SDS–PAGE, the molecular mass of the His6-tagged Gpa3 was 45 kDa. The recombinant protein was recognized by a polyclonal antibody against a fragment of a human Gαi/o/t. Furthermore, the recombinant Gpa3 was ADP-ribosylated by activated cholera toxin and [32P]NAD but not by pertussis toxin. These results indicate that in M. circinelloides the Gα subunit Gpa3 is expressed constitutively during differentiation.Key words: Gα-subunit-encoding genes, Mucor circinelloides, Gpa3 recombinant protein. |
| Databáze: | OpenAIRE |
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