A GC–MS Method for Detection and Quantification of Cathine, Cathinone, Methcathinone and Ephedrine in Oral Fluid
| Autor: | Mahmoud El Said Ali, Khaled M. Mohamed, Alanoud M. Alasiri, Abtehaj H. Al-Hazmi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Cathinone
Phenylpropanolamine 01 natural sciences Methcathinone Gas Chromatography-Mass Spectrometry Analytical Chemistry 03 medical and health sciences Alkaloids 0302 clinical medicine Khat medicine Humans Selected ion monitoring 030216 legal & forensic medicine Ephedrine Saliva Cathine Propiophenones Chromatography biology Illicit Drugs Chemistry 010401 analytical chemistry Reproducibility of Results General Medicine biology.organism_classification 0104 chemical sciences Chemistry Clinical Central Nervous System Stimulants Gas chromatography–mass spectrometry medicine.drug |
| Zdroj: | Journal of Chromatographic Science. 54:1271-1276 |
| ISSN: | 1945-239X 0021-9665 |
| DOI: | 10.1093/chromsci/bmw082 |
| Popis: | The stimulating herbal drug khat (cathine, cathinone) and its analog methcathinone are common substances of abuse in most countries. A GC-MS method was developed and validated for the detection and quantification of cathine, cathinone, methcathinone and ephedrine in oral fluid specimens. The analytes and internal standard (amphetamine-d5) were extracted from 0.5 mL oral fluids by ethyl acetate, and then the dried extracts were derivatized with heptafluorobutyric anhydride at 70°C for 30 min. The MS was used in selected ion monitoring mode. Ions monitored were m/z 117, 240 and 330 for cathine, m/z 77, 105 and 240 for cathinone, m/z 105, 210 and 254 for methcathinone, m/z 210, 254 and 344 for ephedrine and m/z 244 and 336 for amphetamine-d5 The calibration curves were linear (r(2)> 0.98) in the concentration range 20-2,000 ng/mL for all analytes. The intra- and inter-assay imprecisions were within (1.6-12.5%) and (1.5-9.5%), respectively, for all analytes. Intra-assay accuracies were between -5.9 and 6.7% for all analytes. The method was successfully applied to detect and quantify the target analytes from oral fluid specimens collected from Khat and methcathinone users. |
| Databáze: | OpenAIRE |
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