Characterisation of Gel-Forming Mucins Produced In Vivo and In Ex Vivo Conjunctival Explant Cultures
| Autor: | Sara I. Van Acker, Agnė Vailionytė, Zoë P. Van Acker, Bert Van den Bogerd, Carina Koppen, Sorcha Ní Dhubhghaill, Michel Haagdorens, Darlene A. Dartt, Isabel Pintelon |
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| Přispěvatelé: | Ophtalmology - Eye surgery |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male gel-forming mucins Conjunctiva conjunctiva Adolescent goblet cells QH301-705.5 Article Catalysis Tissue Culture Techniques Inorganic Chemistry In vivo medicine Humans Secretion Physical and Theoretical Chemistry Biology (General) Biology Molecular Biology QD1-999 Spectroscopy Aged Aged 80 and over Chemistry Organic Chemistry Transdifferentiation Mucin Mucins General Medicine Middle Aged In vitro Computer Science Applications Cell biology medicine.anatomical_structure Female Gels Ex vivo Explant culture |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 10528, p 10528 (2021) International Journal of Molecular Sciences Volume 22 Issue 19 International journal of molecular sciences |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | One key element to the health of the ocular surface encompasses the presence of gel-forming mucins in the pre-ocular tear film. Conjunctival goblet cells are specialized epithelial cells that secrete mucins necessary for tear film stability and general homeostasis. Their dysfunction can be linked to a range of ocular surface inflammation disorders and chronic injuries. To obtain new perspectives and angles to tackle mucin deficiency, the need for an accurate evaluation of their presence and corresponding mucin secretion in ex vivo conjunctival cultures has become a requisite. In vitro, goblet cells show a significant decrease in the production and secretion of gel-forming mucins, accompanied by signs of dedifferentiation or transdifferentiation. Explant cultures on laminin-treated CLP-PEG hydrogels can, however, support the production of gel-forming mucins. Together, we challenge the current paradigm to evaluate the presence of cultured goblet cells solely based on their general mucin (MUC) content through imaging analyses, showing the need for additional techniques to assess the functionality of goblet cells. In addition, we broadened the gel-forming mucin profile of in vivo goblet cells with MUC5B and MUC6, while MUC2 and MUC6 is added to the profile of cultured goblet cells. ispartof: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES vol:22 issue:19 ispartof: location:Switzerland status: published |
| Databáze: | OpenAIRE |
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