Photoacoustic pigment relocalization sensor
| Autor: | Murad Omar, Antonella Lauri, Dominik Soliman, Ntziachristos, Gil G. Westmeyer, Anja Stelzl |
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| Rok vydání: | 2018 |
| Předmět: |
0303 health sciences
biology Chemistry Xenopus Ligand (biochemistry) biology.organism_classification 01 natural sciences Chromatophore Melanophore 010309 optics Melanin 03 medical and health sciences In vivo 0103 physical sciences Fluorescence microscope Biophysics sense organs 030304 developmental biology Melanosome |
| Popis: | Photoacoustic (optoacoustic) imaging can extract molecular information with deeper tissue penetration than possible by fluorescence microscopy techniques. However, there is currently still a lack of robust genetically controlled contrast agents and molecular sensors that can dynamically detect biological analytes of interest with photoacoustics. In this biomimetic approach, we took inspiration from cuttlefish who can change their color by relocalizing pigment-filled organelles in so-called chromatophore cells under neurohumoral control. Analogously, we tested the use of melanophore cells from Xenopus laevis, containing compartments (melanosomes) filled with strongly absorbing melanin, as whole-cell sensors for optoacoustic imaging. Our results show that pigment relocalization in these cells, which is dependent on binding of a ligand of interest to a specific G protein-coupled receptor (GPCR), can be monitored in vitro and in vivo using photoacoustic mesoscopy. In addition to changes in the photoacoustic signal amplitudes, we could furthermore detect the melanosome aggregation process by a change in the frequency content of the photoacoustic signals. Using bioinspired engineering, we thus introduce a photoacoustic pigment relocalization sensor (PaPiReS) for molecular photoacoustic imaging of GPCR-mediated signaling molecules. |
| Databáze: | OpenAIRE |
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