Identification and transcription control of fission yeast genes repressed by an ammonium starvation growth arrest
| Autor: | Eric Perret, Christine Bonnet, Daniel Caput, Xavier Dumont, Anré Picard, Guy Lenaers |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Ribosomal Proteins
Transcription Genetic Bioengineering Protein Serine-Threonine Kinases Biology Applied Microbiology and Biotechnology Biochemistry Ribosomal protein Schizosaccharomyces Biomarkers Tumor Genetics Transcriptional regulation Protein biosynthesis RNA Messenger Protein kinase A Transcription factor Mitogen-Activated Protein Kinase Kinases Ribosomal Protein S6 Cell growth Calcium-Binding Proteins Tumor Protein Translationally-Controlled 1 Cell cycle biology.organism_classification Cyclic AMP-Dependent Protein Kinases Quaternary Ammonium Compounds Glucose Schizosaccharomyces pombe Schizosaccharomyces pombe Proteins Biotechnology |
| Zdroj: | Yeast. 16:23-33 |
| ISSN: | 1097-0061 0749-503X |
| Popis: | In fission yeast Schizosaccharomyces pombe, ammonium starvation induces a growth arrest, a cell cycle exit in G1 and a further switch to meiosis. This process is regulated by the cAMP-dependent protein kinase and the Wis1-dependent MAP kinase cascade, and downstream transcription factors. In order to understand how cells adapt their genetic programme to the switch from mitotic cycling to starvation, a differential transcript analysis comparing mRNA from exponentially growing and ammonium-starved cells was performed. Genes repressed by this stimulus mainly concern cell growth, i.e. protein synthesis and global metabolism. Comparison of the expression of two of them, the ribosomal proteins Rps6 and TCTP, in many different growing conditions, evidenced a strong correlation, suggesting that their transcriptions are coordinately regulated. Nevertheless, by repeating the ammonium starvation on strains constitutively activated for the PKA pathway (Δcgs1), or unable to activate the Wis1-dependent MAP kinase pathway (Δwis1), or with both characteristics (Δcgs1+Δwis1), the transcriptional inhibition was found to be governed either by the PKA pathway, or by the Wis1 pathway, or by both. These results suggest that during the switch from exponential growth to ammonium starvation, cell homeostasis is maintained by downregulating the transcription of the most expressed genes by a PKA and a Wis1-dependent process. Accession Nos for the S30 and L14 ribosomal protein cDNA sequences are AJ2731 and AJ2732, respectively. Copyright © 2000 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text