Metformin improves cognitive impairment in diabetic mice induced by a combination of streptozotocin and isoflurane anesthesia
| Autor: | Lingxia Zhao, Zhigan Lv, Jianwen Zhang, Wei-Wei Zhang, Pengfei Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
endocrine system diseases Receptor for Advanced Glycation End Products Spatial Learning Bioengineering Hippocampal formation medicine.disease_cause Hippocampus Applied Microbiology and Biotechnology streptozotocin diabetic mice Streptozocin Diabetes Mellitus Experimental isoflurane Western blot Memory medicine Animals Hippocampus (mythology) Memory impairment Anesthesia Cognitive Dysfunction cognitive impairment medicine.diagnostic_test business.industry NF-kappa B nutritional and metabolic diseases General Medicine Streptozotocin Metformin Mice Inbred C57BL Disease Models Animal Oxidative Stress Isoflurane business metformin Oxidative stress TP248.13-248.65 Signal Transduction Research Article Research Paper medicine.drug Biotechnology |
| Zdroj: | Bioengineered, Vol 12, Iss 2, Pp 10982-10993 (2021) Bioengineered article-version (VoR) Version of Record |
| ISSN: | 2165-5987 2165-5979 |
| Popis: | To investigate the protective effects of metformin on the diabetic mice with cognitive impairment induced by the combination of streptozotocin (STZ) and isoflurane anesthesia. The isoflurane-anesthetized cognitive impairment model mice were established and then observed via behavioral tests and histopathological examination. Then these model mice were randomly assigned to three groups, which received the PBS, low and high doses of metformin, respectively. The body weight, food and water consumption of model mice were measured every other day. The mechanisms of metformin on ameliorating the cognitive dysfunction were further investigated by histomorphological, biochemical and Western blot analysis. After 14-days treatment of metformin, the diabetic symptoms in STZ-induced diabetic mice were significantly alleviated. Metformin could restore the isoflurane- and STZ-induced hippocampal tissue damage, cognitive and memory impairment in exposed space via improving the oxidative stress, upregulating the contents of glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) in the hippocampus tissues of diabetic mice. Furthermore, chronic treatment of metformin significantly down-regulated the expression of AGEs, RAGE, pNF-κB, iNOS, and COX-2. In conclusion, metformin can improve the isoflurane- and STZ-induced cognitive impairment in diabetic mice via improving oxidative stress and inhibiting the AGEs/RAGE/NF-κB signaling pathway. |
| Databáze: | OpenAIRE |
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