The expression of the imprinted genes H19 and IGF-2 in choriocarcinoma cell lines. Is H19 a tumor suppressor gene?
| Autor: | O Lustig-Yariv, Avraham Hochberg, E Schulze, Volker A. Erdmann, Dymitr Komitowski, T Schneider, N. de Groot |
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| Rok vydání: | 1997 |
| Předmět: |
Cancer Research
RNA Untranslated Tumor suppressor gene Mice Nude Muscle Proteins Biology medicine.disease_cause Transfection 03 medical and health sciences Mice 0302 clinical medicine Insulin-Like Growth Factor II Genetics medicine Tumor Cells Cultured Animals Humans Genes Tumor Suppressor Choriocarcinoma Molecular Biology Gene 030304 developmental biology 0303 health sciences Expression vector Chromosome Neoplasms Experimental Molecular biology female genital diseases and pregnancy complications Cell culture 030220 oncology & carcinogenesis embryonic structures Cancer research RNA Long Noncoding Genomic imprinting Carcinogenesis Cell Division |
| Zdroj: | Oncogene. 15(2) |
| ISSN: | 0950-9232 |
| Popis: | H19 is a paternally imprinted gene with unknown function. It is located in close proximity to the maternally imprinted IGF-2 gene on chromosome 11p15.5. In this study no consistent relationship between the expression of these two genes in clones derived from JEG-3 and JAr cell lines could be detected. Nor could a consistent relationship be detected between the expression levels of these two genes and between certain characteristic tumorigenic properties of these clones. We included in this study clones, expressing low H19 levels, which after transfection with an H19 expression construct highly expressed the H19 gene. In tumors, formed by the injection of cells of JAr or JEG-3 clones into nude mice, the H19 expression was high and irrelevant to the expression level in the cells before the injection. The same phenomenon was found for IGF-2 expression during tumorigenesis caused by cells of different JEG-3 clones and in some but not all JAr derived clones. Both H19 and IGF-2 are biallelicly expressed in all the JAr and JEG-3 clones. In summary, our observations point to the conclusion that H19 is not a tumor suppressor gene. However, its high expression in all the tumors formed after injection of cells of the JAr and JEG-3 clones, leaves its role, if any, in choriocarcinogenesis an open question. |
| Databáze: | OpenAIRE |
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