Self-standardization of quality of bacterial cellulose produced by Medusomyces gisevii in nutrient media derived from Miscanthus biomass
| Autor: | Ekaterina А. Skiba, V. V. Budaeva, Gennady V. Sakovich, Lyudmila А. Aleshina, Evgenia K. Gladysheva, Dmitri S. Golubev |
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| Rok vydání: | 2020 |
| Předmět: |
Polymers and Plastics
Biomass 02 engineering and technology Raw material 010402 general chemistry Poaceae 01 natural sciences chemistry.chemical_compound Crystallinity Nutrient Enzymatic hydrolysis Materials Chemistry Food science Cellulose biology Bacteria Hydrolysis Organic Chemistry Miscanthus 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Culture Media chemistry Bacterial cellulose Yield (chemistry) Saccharomycetales 0210 nano-technology Biotechnology |
| Zdroj: | Carbohydrate polymers. 252 |
| ISSN: | 1879-1344 |
| Popis: | Bacterial cellulose (BC) was synthesized from biomass of Miscanthus grown in West Siberia. Miscanthus biomass was pretreated at atmospheric pressure with 4 wt.% solutions of HNO3 and NaOH in one and two stages. The effect of four methods of the pretreatment of the feedstock on BC yield and properties was examined. The resultant pulps were subjected to enzymatic hydrolysis with commercial CelloLux-A and BrewZyme BGX enzymes. Biosynthesis of BC was run under static and non-sterile conditions using Medusomyces gisevii Sa-12. The two-stage pretreatment of Miscanthus biomass gave a 20 % increase in BC production compared to the single-stage pretreatment. The resultant BC exhibited a high crystallinity index (88–93 %) and an extraordinarily high content of allomorph Iα (99–100 %), irrespective of the pretreatment method; therefore, it has been revealed for the first time that the Medusomyces gisevii Sa-12 symbiotic culture is capable of self-standardization against the quality of produced BC. |
| Databáze: | OpenAIRE |
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