Glassy droplet inclusions within the cytoplasm of Kupffer cells: A novel ultrastructural feature for the diagnosis of pediatric autoimmune hepatitis
| Autor: | Joanna Maria Lotowska, Dariusz Marek Lebensztejn, Maria Elzbieta Sobaniec-Lotowska, Urszula Daniluk |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Hyalin Pathology medicine.medical_specialty Adolescent Kupffer Cells Autoimmune hepatitis Mitochondrion Cytoplasmic droplets Pathogenesis 03 medical and health sciences 0302 clinical medicine immune system diseases Fibrosis medicine Humans Child skin and connective tissue diseases Inclusion Bodies Hepatology business.industry Gastroenterology Ultrastructural feature medicine.disease digestive system diseases eye diseases Hepatitis Autoimmune Liver Cytoplasm Child Preschool 030220 oncology & carcinogenesis Ultrastructure Female 030211 gastroenterology & hepatology Poland business |
| Zdroj: | Digestive and Liver Disease. 49:929-933 |
| ISSN: | 1590-8658 |
| DOI: | 10.1016/j.dld.2017.04.001 |
| Popis: | Since Kupffer cells/macrophages (KCs/MPs) may be involved in the pathogenesis of autoimmune hepatitis (AIH), this pioneer study was undertaken to evaluate KCs/MPs in pediatric AIH in transmission-electron microscope. Methods Ultrastructural analyses were performed using liver biopsies from 14 children with clinicopathologically diagnosed AIH. Results In all AIH children, ultrastructural findings revealed changes in the cells lining sinusoidal vessels, especially KCs/MPs and endothelial cells. KCs/MPs showed increased phagocytic activity and damaged mitochondria, frequently with accompanying intense fibrosis. In 10/14 AIH patients, the cytoplasm of sinusoidal KCs/MPs contained characteristic glassy droplet inclusions. They were round, sharply circumscribed, and contained homogeneous material and distinct translucent fields. Their ultrastructure was identical with the Russel bodies of plasma cells, which were also found in liver biopsies in the same patients. Conclusion Ultrastructural identification of characteristic cytoplasmic droplets with glassy appearance in KCs/MPs, never before described in AIH, provides a useful novel morphological feature in the diagnosis of this disease. |
| Databáze: | OpenAIRE |
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