Protein surface shielding agents in protein crystallization
| Autor: | J. Hašek |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Nuclear and High Energy Physics
Diffraction Structural Biology Surface Properties law.invention Polyethylene Glycols Crystal Protein structure X-Ray Diffraction law Molecule Crystallization Instrumentation Radiation intermolecular interactions Chemistry Intermolecular force Proteins Adhesion Crystallography competitive adhesion modes X-ray crystallography Biophysics protein surface shielding agents polymer crystallization screens Protein crystallization Hydrophobic and Hydrophilic Interactions protein adhesion |
| Zdroj: | Journal of Synchrotron Radiation |
| ISSN: | 1600-5775 |
| Popis: | The crystallization process can be controlled by protein surface shielding agents blocking undesirable competitive adhesion modes during non-equilibrium processes of deposition of protein molecules on the surface of growing crystalline blocks. The hypothesis is based on a number of experimental proofs from diffraction experiments and also retrieved from the Protein Data Bank. The molecules adhering temporarily on the surface of protein molecules change the propensity of protein molecules to deposit on the crystal surface in a definite position and orientation. The concepts of competitive adhesion modes and protein surface shielding agents acting on the surface of molecules in a non-equilibrium process of protein crystallization provide a useful platform for the control of crystallization. The desirable goal, i.e. a transient preference of a single dominating adhesion mode between protein molecules during crystallization, leads to uniform deposition of proteins in a crystal. This condition is the most important factor for diffraction quality and thus also for the accuracy of protein structure determination. The presented hypothesis is a generalization of the experimentally well proven behaviour of hydrophilic polymers on the surface of protein molecules of other compounds. |
| Databáze: | OpenAIRE |
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