Slits affect the timely migration of neural crest cells via robo receptor
| Autor: | Maria Elena de Bellard, Darwin Martinez, Joshua Kaiser, Rachel Reyes, Mary-Pat Stein, Lisa Correa, Le Ma, Gustavo Gomez, Hannah Ra, Michelle Reyes, Dion Giovannone |
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| Rok vydání: | 2012 |
| Předmět: |
animal structures
Nerve Tissue Proteins Chick Embryo Biology Time-Lapse Imaging Article Cell Movement medicine Animals Receptors Immunologic Cells Cultured Cytoskeleton In Situ Hybridization Neural fold Microscopy Video Reverse Transcriptase Polymerase Chain Reaction Neural tube Neural crest Cell Differentiation Cell migration Slit Cell biology medicine.anatomical_structure Trunk neural crest cell migration Neural Crest embryonic structures Intercellular Signaling Peptides and Proteins Neural crest cell migration Chickens Neural plate Developmental Biology |
| Zdroj: | Developmental Dynamics. 241:1274-1288 |
| ISSN: | 1058-8388 |
| DOI: | 10.1002/dvdy.23817 |
| Popis: | Background: Neural crest cells emerge by delamination from the dorsal neural tube and give rise to various components of the peripheral nervous system in vertebrate embryos. These cells change from non-motile into highly motile cells migrating to distant areas before further differentiation. Mechanisms controlling delamination and subsequent migration of neural crest cells are not fully understood. Slit2, a chemorepellant for axonal guidance that repels and stimulates motility of trunk neural crest cells away from the gut has recently been suggested to be a tumor suppressor molecule. The goal of this study was to further investigate the role of Slit2 in trunk neural crest cell migration by constitutive expression in neural crest cells. Results: We found that Slit gain-of-function significantly impaired neural crest cell migration while Slit loss-of-function favored migration. In addition, we observed that the distribution of key cytoskeletal markers was disrupted in both gain and loss of function instances. Conclusions: These findings suggest that Slit molecules might be involved in the processes that allow neural crest cells to begin migrating and transitioning to a mesenchymal type. |
| Databáze: | OpenAIRE |
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