Highly frequent allelic loss of chromosome 6q16-23 in osteosarcoma: involvement of cyclin C in osteosarcoma
| Autor: | Aki Yoshida, Kenji Shimizu, Sachio Ito, Mamoru Ouchida, Kunihiko Numoto, Yoshimi Jitsumori, Norihide Ohata, Toshiyuki Kunisada, Toshifumi Ozaki, Hirotaka Kanzaki |
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| Rok vydání: | 2006 |
| Předmět: |
Genetic Markers
DNA Complementary Tumor suppressor gene Loss of Heterozygosity Biology Loss of heterozygosity Cyclin C Cell Line Tumor Cyclins Genetics Humans RNA Messenger Osteosarcoma Physical Chromosome Mapping Chromosome Nucleic Acid Hybridization General Medicine Molecular biology Candidate Tumor Suppressor Gene Genetic marker Cancer research Chromosomes Human Pair 6 CCNC Gene Deletion Comparative genomic hybridization Microsatellite Repeats |
| Zdroj: | International journal of molecular medicine. 18(6) |
| ISSN: | 1107-3756 |
| Popis: | The molecular pathogenesis of osteosarcoma is very complicated and associated with chaotic abnormalities on many chromosomal arms. We analyzed 12 cases of osteosarcomas with comparative genomic hybridization (CGH) to identify chromosomal imbalances, and detected highly frequent chromosomal alterations in chromosome 6q, 8p, 10p and 10q. To define the narrow rearranged region on chromosome 6 with higher resolution, loss of heterozygosity (LOH) analysis was performed with 21 microsatellite markers. Out of 31 cases, 23 cases (74%) showed allelic loss at least with one marker on chromosome 6q. We identified two distinct commonly deleted regions on chromosome 6 using markers D6S1565 located at 6q16 and 6q23MS1 at 6q23. The expression analysis of genes located at the deleted region was performed, and the decreased mRNA expression of the CCNC gene, one of the regulators of cell cycle, was detected. Growth of osteosarcoma cell line was significantly suppressed after the CCNC cDNA transfection. Fine mapping of the deleted region containing a possible tumor suppressor gene and the transfection assay suggest that the CCNC is a candidate tumor suppressor gene. |
| Databáze: | OpenAIRE |
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