Whole genome survey of big cats (Genus: Panthera) identifies novel microsatellites of utility in conservation genetic study
| Autor: | Damdingiin Bayarkhagva, Anna Vitkalova, Randeep Singh, Jee Yun Hyun, Alvin Chon, Kyung Seok Kim, Puneet Pandey, Surendra Prakash Goyal, Taisia Marchenkova, Mi-Sook Min, Daecheol Jeong, Jong Bhak, Hye Kyung Song, Mihyeon Yu, Hang Lee |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetic Markers Conservation genetics Conservation of Natural Resources Molecular biology Science Wildlife 010603 evolutionary biology 01 natural sciences Genome Article 03 medical and health sciences Surveys and Questionnaires biology.animal Genetics Animals Panthera Probability Apex predator Multidisciplinary Polymorphism Genetic Base Sequence biology Eurasian lynx Biological techniques Big cat Computational biology and bioinformatics 030104 developmental biology Evolutionary biology Threatened species Microsatellite Medicine Microsatellite Repeats |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Big cats (Genus: Panthera) are among the most threatened mammal groups of the world, owing to illegal transnational trade. Conservation genetic studies and effective curbs on poaching are important for the conservation of these charismatic apex predators. A limited number of microsatellite markers exists for Panthera species and researchers often cross-amplify domestic cat microsatellites to study these species. We conducted data mining of seven Panthera genome sequences to discover microsatellites for conservation genetic studies of four threatened big cat species. A total of 32 polymorphic microsatellite loci were identified in silico and tested with 99 big cat individuals and 7 Eurasian lynx. The developed markers were polymorphic in most of the tested species. We propose a set of 15 novel microsatellite markers for use in conservation genetics and wildlife forensic investigations of big cat species. Cumulatively, these markers have a high discriminatory power of one in a million for unrelated individuals and one in a thousand for siblings. Similar PCR conditions of these markers increase the prospects of achieving efficient multiplex PCR assays. This study is a pioneering attempt to synthesise genome wide microsatellite markers for big cats. |
| Databáze: | OpenAIRE |
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