BP1, a new homeobox gene, is frequently expressed in acute leukemias

Autor: Patricia E. Berg, Sidney W. Fu, Donna M. Williams, B. D. Smith, Judith E. Karp, FW Ruscetti, Frederick G. Behm, David L. Becton, Susanne B. Haga, Douglas D. Ross, Myron Chang, W. D. Hankins, Susana C. Raimondi
Rok vydání: 2000
Předmět:
Cancer Research
DNA
Complementary
Cellular differentiation
Recombinant Fusion Proteins
Molecular Sequence Data
Biology
environment and public health
hemic and lymphatic diseases
Acute lymphocytic leukemia
medicine
Biomarkers
Tumor
Humans
Protein Isoforms
RNA
Messenger
RNA
Neoplasm
CDX2
Tumor Stem Cell Assay
Homeodomain Proteins
Oncogene Proteins
Leukemia
Gene Expression Regulation
Leukemic
Reverse Transcriptase Polymerase Chain Reaction
fungi
Age Factors
Genes
Homeobox
Myeloid leukemia
Gene Expression Regulation
Developmental
Bone Marrow Examination
Cell Differentiation
Hematology
DNA
Neoplasm
medicine.disease
Hematopoietic Stem Cells
Neoplasm Proteins
enzymes and coenzymes (carbohydrates)
homeobox A9
Alternative Splicing
medicine.anatomical_structure
Cell Transformation
Neoplastic
Oncology
Acute Disease
Cancer research
Ectopic expression
Bone marrow
biological phenomena
cell phenomena
and immunity
K562 Cells
Transcription Factors
Zdroj: Leukemia. 14(11)
ISSN: 0887-6924
Popis: Aberrant expression of homeobox genes has been described in primary leukemia blasts. We recently cloned a new cDNA, BP1, which is a member of the homeobox gene family. BP1 expression was investigated in bone marrow samples from acute myeloid leukemia (AML), acute T cell lymphocytic leukemia (ALL) and pre-B cell ALL. Expression levels of two apparent isoforms of BP1, DLX7 and DLX4, were measured in the same samples. They are weakly if at all detectable in normal bone marrow, PHA-stimulated T cells or B cells. BP1 RNA was highly expressed in 63% of AML cases, including 81% of the pediatric and 47% of the adult cases, and in 32% of T-ALL cases, but was not found in any of the pre-B ALL cases. Coexpression of BP1, DLX7 and DLX4 occurred in a significant number of leukemias. Our data, including co-expression of BP1 with c-myb and GATA-1, markers of early progenitors, suggest that BP1 expression occurs in primitive cells in AML. Analysis of CD34+ and CD34- normal bone marrow cells revealed BP1 is expressed in CD34- cells and virtually extinguished in CD34+ cells. Ectopic expression of BP1 in the leukemia cell line K562 increased clonogenicity, consistent with a role for BP1 in leukemogenesis. The presence of BP1 RNA in leukemic blasts may therefore be a molecular marker for primitive cells and/or may indicate that BP1 is an important upstream factor in an oncogenic pathway.
Databáze: OpenAIRE
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