Genetic diversity of Streptomyces spp. causing common scab of potato in eastern Canada
Autor: | Renée St-Onge, Claudia Goyer, Martin Filion, Robert Coffin |
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Rok vydání: | 2008 |
Předmět: |
Canada
Genomic Islands Molecular Sequence Data Polymerase Chain Reaction Applied Microbiology and Biotechnology Microbiology Streptomyces Bacterial Proteins RNA Ribosomal 16S Genetic variation Ecology Evolution Behavior and Systematics Plant Diseases Solanum tuberosum Melanins Genetics Genetic diversity Polymorphism Genetic biology Common scab Genetic Variation Ribosomal RNA biology.organism_classification Streptomyces scabies rpoB DNA Fingerprinting Plant Tubers Phenotype Genes Bacterial Streptomyces acidiscabies |
Zdroj: | Systematic and Applied Microbiology. 31:474-484 |
ISSN: | 0723-2020 |
Popis: | Common scab is an important disease of potato caused by Streptomyces scabies and other closely related species. In this study, the genetic diversity of Streptomyces spp. causing common scab of potato in eastern Canada was for the first time investigated. Forty-one Streptomyces spp. isolates were retrieved from necrotic lesions of potato tubers harvested from different regions of the Canadian provinces New-Brunswick, Nova Scotia and Prince-Edward-Island. Most isolates were closely related to known pathogenic S. scabies strains on the basis of partial 16S ribosomal (r) RNA and rpoB gene sequence analyses. Two isolates were identified as pathogenic species of Streptomyces acidiscabies. To our knowledge, this species has never been previously isolated in these areas. Genome fingerprinting studies using repetitive elements (rep) polymerase chain reactions (PCR) revealed 10 distinct genetic groups in eastern Canada. The geographical distribution of the genetic groups was region-dependant. Pathogenicity- and virulence-related genes (txtA, txtC, and tomA) were PCR-amplified from each isolate, and nucleotide sequence analysis of partial gene fragments revealed slight polymorphisms in both txtA and txtC genes. No genetic variation was noted in the partial tomA gene sequences. |
Databáze: | OpenAIRE |
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