Bio-molecule functionalized rapid one-pot green synthesis of silver nanoparticles and their efficacy toward the multidrug resistant (MDR) gut bacteria of silkworms (Bombyx mori)
| Autor: | Ismail Ocsoy, A. K. Deb, Sukhendu Mandal, Soumen Saha, Amitava Mandal, Abdul Sadat, Kinkar Biswas, Debjoy Bhattacharjya, Amit Kumar Mandal, Octavio L. Franco, Ahmet Kati, Tushar K. Jana, Biraj Sarkar, Sudip Some, Anoop Kumar, İkbal Agah İnce, Paulami Dam, Rittick Mondal |
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| Přispěvatelé: | Acibadem University Dspace |
| Rok vydání: | 2020 |
| Předmět: |
biology
General Chemical Engineering 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences Silver nanoparticle 0104 chemical sciences Multiple drug resistance chemistry.chemical_compound chemistry Zeta potential MTT assay Gallic acid Surface plasmon resonance 0210 nano-technology Cytotoxicity Bacteria Nuclear chemistry |
| Popis: | The present study aimed to synthesise bio-molecule functionalized silver nanoparticles (AgNPs) using leaf extract from mulberry variety S-1635 (Morus alba L.) and to explore its antibacterial efficacy against multidrug resistant (MDR) gut bacteria isolated from natural infection observed from silkworm larvae in rearing conditions. AgNPs formation was established by surface plasmon resonance at 480 nm. The crystallinity of the synthesised AgNPs was checked by HR-TEM and XRD analysis. SEM and TEM characterisation further exhibited the spherical, monodispersed, well scattered nature of the AgNPs with an average particle size of 11.8 nm ± 2.8. The presence of (111), (200), (220) and (311) planes in Bragg's reflections confirmed the face-cantered-cubic crystalline silver. EDX analysis confirmed the presence of elemental silver. FT-IR spectra revealed functional groups were responsible for the reduction of silver ions. The zeta potential value of −17.3 mV and −25.6 mV was recorded in MH and DMEM/F-12 media, respectively. The LC-QTOF/MS and HRMS spectra disclosed the presence of bioactive compounds like flavonoid, gallic acid, and stigmasterol, which are probably involved in the reduction and functionalization of AgNPs. The antibacterial efficacy of bio-molecule functionalized AgNPs and the naked AgNPs was tested on Gram-positive and Gram-negative bacteria isolated from silkworms and characterized by using 16S rDNA and gyrB genes. The cytotoxicity of AgNPs was tested on WRL-68, HEK-293, ACHN, and HUH-7 cell lines using MTT assay. This study provides an insight into the application of bio-molecule functionalized AgNPs for combating various silkworm pathogens which severely affect the agro-rural economy of developing countries. |
| Databáze: | OpenAIRE |
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