Structural and mechanistic insights into the substrate specificity and hydrolysis of GH31 α-N-acetylgalactosaminidase
| Autor: | Marina Ikegaya, Takatsugu Miyazaki, Santiago Alonso-Gil |
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| Rok vydání: | 2022 |
| Předmět: |
Glycoside Hydrolases
Stereochemistry N-acetylgalactosamine Tn antigen Peptide Quantum mechanics Biochemistry Substrate Specificity alpha-N-Acetylgalactosaminidase N-Acetylgalactosamine chemistry.chemical_compound Catalytic Domain Glycoside hydrolase family 31 Gut bacteria chemistry.chemical_classification biology Hydrolysis Active site General Medicine Fetuin O-glycan Enzyme chemistry Docking (molecular) Mucin biology.protein |
| Zdroj: | Biochimie. 195:90-99 |
| ISSN: | 0300-9084 |
| DOI: | 10.1016/j.biochi.2021.11.007 |
| Popis: | Glycoside hydrolase family 31 (GH31) is a diversified family of anomer-retaining α-glycoside hydrolases, such as α-glucosidase and α-xylosidase, among others. Recently, GH31 α-N-acetylgalactosaminidases (Nag31s) have been identified to hydrolyze the core of mucin-type O-glycans and the crystal structure of a gut bacterium Enterococcus faecalis Nag31 has been reported. However, the mechanisms of substrate specificity and hydrolysis of Nag31s are not well investigated. Herein, we show that E. faecalis Nag31 has the ability to release N-acetylgalactosamine (GalNAc) from O-glycoproteins, such as fetuin and mucin, but has low activity against Tn antigen. Mutational analysis and crystal structures of the Michaelis complexes reveal that residues of the active site work in concert with their conformational changes to act on only α-N-acetylgalactosaminides. Docking simulations using GalNAc-attached peptides suggest that the enzyme mainly recognizes GalNAc and side chains of Ser/Thr, but not strictly other peptide residues. Moreover, quantum mechanics calculations indicate that the enzyme preferred p-nitrophenyl α-N-acetylgalactosaminide to Tn antigen and that the hydrolysis progresses through a conformational itinerary, 4C1 → 1S3 → 4C1, in GalNAc of substrates. Our results provide novel insights into the diversification of the sugar recognition and hydrolytic mechanisms of GH31 enzymes. |
| Databáze: | OpenAIRE |
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