Investigations of Bacterial Inactivation and DNA Fragmentation Induced by Flowing Humid Argon Post-discharge
| Autor: | B. Dodet, Soukayna Limam, Michael J. Kirkpatrick, Emmanuel Odic, M. S. DuBow, S. Salamitou |
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| Přispěvatelé: | Supélec Sciences des Systèmes (E3S), Ecole Supérieure d'Electricité - SUPELEC (FRANCE), Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Z. Machala, Karol Hensel and Yuri Akishev |
| Rok vydání: | 2011 |
| Předmět: |
010302 applied physics
Aqueous solution Argon Atmospheric pressure chemistry.chemical_element 02 engineering and technology Plasma Dielectric barrier discharge 021001 nanoscience & nanotechnology Photochemistry 01 natural sciences 6. Clean water Dissociation (chemistry) [SPI]Engineering Sciences [physics] chemistry.chemical_compound chemistry 0103 physical sciences DNA fragmentation 0210 nano-technology Hydrogen peroxide |
| Zdroj: | Plasma for Bio-Decontamination, Medicine and Food Security ISBN: 9789400728516 Plasma for Bio-Decontamination, Medicine and Food Security Z. Machala, Karol Hensel and Yuri Akishev. Plasma for Bio-Decontamination, Medicine and Food Security, Kluwer Academic Publisher, Chapter 8 ; pp. 93-106, 2012, NATO Science for Peace and Security Series A: Chemistry and Biology, ⟨10.1007/978-94-007-2852-3_8⟩ |
| DOI: | 10.1007/978-94-007-2852-3_8 |
| Popis: | Bio-contaminated surfaces were exposed to an atmospheric pressure flowing post-discharge, i.e. without direct contact of the plasma with the surface. The non-thermal plasma source was a dielectric barrier discharge. Using humid argon as a feed gas, a reduction of six orders of magnitude of survivors could be obtained for Escherichia coli. An investigation of bacterial inactivation mechanisms during the plasma induced treatment was conducted. For this purpose, DNA (plasmid and genomic DNA in aqueous solution) degradation by the plasma process was studied, assuming that the bacterial inactivation is obtained when the bacterial DNA is fragmented. According to the operating conditions (feed gas, reactor geometry and discharge input power), DNA fragmentation was evaluated in correlation with aqueous phase hydrogen peroxide concentration measurements. It appears that hydrogen peroxide is not the only factor responsible for DNA fragmentation and that short-lived species produced by water dissociation are major contributors. |
| Databáze: | OpenAIRE |
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