Pharmacokinetics of flumequine and in vitro activity against bacterial pathogens of gilthead sea bream Sparus aurata
| Autor: | George Rigos, I. Nengas, Maria N. Alexis, G. M. Troisi, Maria Koutsodimou, A.E. Tyrpenou, Maria Yiagnisis |
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| Rok vydání: | 2003 |
| Předmět: |
Vibrio anguillarum
Sparidae Administration Oral Biological Availability Aquaculture Microbial Sensitivity Tests Aquatic Science Microbiology Minimum inhibitory concentration Fish Diseases Pharmacokinetics medicine Animals Tissue Distribution Food science Ecology Evolution Behavior and Systematics Antibacterial agent Vibrio biology Dose-Response Relationship Drug Photobacterium Biological activity biology.organism_classification Sea Bream Bioavailability Anti-Bacterial Agents Treatment Outcome Vibrio Infections Flumequine Injections Intravenous Quinolizines medicine.drug Fluoroquinolones |
| Zdroj: | Diseases of aquatic organisms. 54(1) |
| ISSN: | 0177-5103 |
| Popis: | The present study investigated the kinetic profile of flumequine (FLU) in gilthead sea bream Sparus aurata (170 g) held at 19 degrees C and evaluated its in vitro efficacy against important bacterial diseases in Mediterranean mariculture. Following a single intravascular injection (10 mg kg(-1) fish), the distribution half-life (t1/2alpha) and the half-life of the terminal phase of elimination (t1/2gamma) of the drug were 0.2 and 30 h respectively. Tissue penetration of FLU was low, since both the apparent distribution volume of the drug at steady-state (Vd(SS)) and the apparent volume of the central compartment (Vc) were small (0.57 and 0.15 l kg(-1)). The mean residence time (MRT) was short (11 h) and the total clearance (CL(T)) of the drug was slow (0.05 l kg(-1) h(-1)). Following oral administration (20 mg kg(-1)), the bioavailability (F %) of FLU was 29% and the maximum plasma concentration was 1.7 microg ml(-1). The minimum inhibitory concentration (MIC) of the drug in distilled water supplemented with 2% NaCl against Vibrio anguillarum Serotype 1b, Photobacterium damsela ssp. piscicida, V. alginolyticus, V. damsela and V. fluvialis was 0.15, 0.3, 1.2, 0.019 and 0.15 microg ml(-1) respectively. The addition however of 10 mM Ca2+ and 55 mM Mg2+ to the medium resulted in an 8- to >120-fold reduction in FLU activity. The results indicate that FLU has an adequate kinetic profile in gilthead sea bream and that marine cations induce a significant impact on the activity of FLU, rendering its use against bacterial pathogens questionable. |
| Databáze: | OpenAIRE |
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