Modulation of human T cell cytokines by the Mycobacterium tuberculosis-secreted protein Wag31
| Autor: | Malini Rajagopalan, Na Yi, Buka Samten, Krishna Sarva, Murty V. V. S. Madiraju, Stewart Fannin |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) medicine.drug_class T cell T-Lymphocytes 030106 microbiology Immunology Monoclonal antibody Lymphocyte Activation Microbiology Mycobacterium tuberculosis 03 medical and health sciences Interferon-gamma Structure-Activity Relationship Immune system Antigen Bacterial Proteins Protein Domains Extracellular medicine Cytotoxic T cell Humans Secretion Cells Cultured biology Dose-Response Relationship Drug Interleukin-17 biology.organism_classification Interleukin-10 030104 developmental biology Infectious Diseases medicine.anatomical_structure Cytokines |
| Zdroj: | Tuberculosis (Edinburgh, Scotland). |
| ISSN: | 1873-281X |
| Popis: | Mycobacterium tuberculosis secretes a number of proteins into the extracellular milieu during growth. Several of these proteins have been associated with modulation of the host immune response. Antigen 84, or Wag31, is one such protein that is conserved among all mycobacterial species and is recognized by the sera from tuberculosis and leprosy patients. Here, we examined the effect of Wag31 on the ability of activated human T cells to produce cytokines such as IL-10, IL-17 and IFN-γ in response to combined anti-CD3 and anti-CD28 stimulation. Purified recombinant Wag31 inhibited the secretion of IL-10 and IL-17, but not IFN-γ, by human T cells stimulated with plate-bound anti-CD3 and anti-CD28 monoclonal antibodies. Furthermore, the C-terminal domain, but not the N-terminal domain, inhibited the production of IL-10 and IL-17 without a significant effect on the production of IFN-γ. These data suggest that Wag31 may modulate human T cell immune responses during tuberculosis infection through its C-terminal domain. |
| Databáze: | OpenAIRE |
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