Brewster Angle Microscopy and PMIRRAS Study of DNA Interactions with BGTC, a Cationic Lipid Used for Gene Transfer
| Autor: | Pierre Lehn, Lehn Jean-Marie, Annie Février, Bernard Desbat, Brigitte Delord, Sabine Castano |
|---|---|
| Přispěvatelé: | Chimie et Biologie des Membranes et des Nanoobjets (CBMN), Université de Bordeaux (UB)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre de recherches Paul Pascal (CRPP), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie des Interactions Moléculaires, Chaire Chimie des Interactions Moléculaires, Collège de France (CdF (institution)), Génétique moléculaire et génétique épidémiologique, Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Brest (UBO) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
DNA complexes
Time Factors Spectrophotometry Infrared Surface Properties Genetic Vectors Lipid Bilayers Analytical chemistry Supramolecular chemistry Biophysics 02 engineering and technology Cationic Lipid Guanidines 03 medical and health sciences symbols.namesake Brewster Angle Microscopy Cations Monolayer Electrochemistry Pressure [CHIM]Chemical Sciences General Materials Science Lipid bilayer Spectroscopy 030304 developmental biology 0303 health sciences Liposome Microscopy Brewster's angle Chemistry Bilayer Air Cationic polymerization Gene Transfer Techniques Water Surfaces and Interfaces DNA 021001 nanoscience & nanotechnology Condensed Matter Physics Lipids Crystallography Cholesterol symbols lipids (amino acids peptides and proteins) Self-assembly 0210 nano-technology |
| Zdroj: | Langmuir Langmuir, American Chemical Society, 2008, 24 (17), pp. 9598-9606. ⟨10.1021/la703491r⟩ |
| ISSN: | 0743-7463 1520-5827 |
| DOI: | 10.1021/la703491r⟩ |
| Popis: | 9 pages; International audience; The lipid bis(guanidinium)-tris(2-aminoethyl)amine-cholesterol (BGTC) is a cationic cholesterol derivative bearing guanidinium polar headgroups which displays high transfection efficiency in Vitro and in ViVo when used alone or formulated as liposomes with the neutral colipid 1,2-di-[cis-9-octadecenoyl]-sn-glycero-3-phosphoethanolamine (DOPE). Since transfection may be related to the structural and physicochemical properties of the self-assembled supramolecular lipid-DNA complexes, we used the Langmuir monolayer technique coupled with Brewster angle microscopy (BAM) and polarization modulation infrared reflection absorption spectroscopy (PMIRRAS) to investigate DNA-BGTC and DNA-BGTC/DOPE interactions at the air/water interface. We herein show that BGTC forms stable monolayers at the air/water interface. When DNA is injected into the subphase, it adsorbs to BGTC at 20 mN/m. Whathever the (+/-) charge ratio of the complexes used, defined as the ratio of positive charges of BGTC in the monolayer versus negative charges of DNA injected in the subphase, the DNA interacts with the cationic lipid and forms either an incomplete (no constituent in excess) or a complete (DNA in excess) monolayer of oriented double strands parallel to the lipid monolayer plan. We also show that, under a homogeneous BGTC/DOPE (3/2) monolayer at 20 mN/m, DNA adsorbs homogeneously to form an organized but incomplete layer whatever the charge ratio used (DNA in default or in excess). Compression beyond the collapse of these mixed DNA-BGTC/DOPE systems leads to the formation of dense DNA monolayers under an asymmetric lipid bilayer with a bottom layer of BGTC in contact with DNA and a top layer mainly constituted of DOPE. These results allow a better understanding of the mechanisms underlying the formation of the supramolecular BGTC-DNA complexes efficient for gene transfection. |
| Databáze: | OpenAIRE |
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