1,2:3,4-Diepoxybutane Induces Multipolar Mitosis in Cultured Human Lymphocytes
| Autor: | César Borjas Gutiérrez, Martín Daniel Domínguez Cruz, María de Lourdes Ramírez Dueñas, Reyna Lucía Barajas Torres, María Teresa Magaña Torres, Juan Ramón González García |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Lymphocyte Mitomycin Aneuploidy Diepoxybutane Mitosis Biology 03 medical and health sciences Clastogen chemistry.chemical_compound Fanconi anemia Toxicity Tests Genetics medicine Butadienes Sister chromatids Humans Lymphocytes Molecular Biology Genetics (clinical) Carcinogen Cells Cultured medicine.disease Molecular biology Healthy Volunteers 030104 developmental biology medicine.anatomical_structure chemistry Immunology Micronucleus test Epoxy Compounds Mutagens |
| Zdroj: | Cytogenetic and genome research. 148(2-3) |
| ISSN: | 1424-859X |
| Popis: | 1,3-Butadiene, a colorless gas regularly used in the production of plastics, thermoplastic resins, and styrene-butadiene rubber, poses an increased leukemia mortality risk to workers in this field. 1,3-Butadiene is also produced by incomplete combustion of motor fuels or by tobacco smoking. It is absorbed principally through the respiratory system and metabolized by several enzymes rendering 1,2:3,4-diepoxybutane (DEB), which has the highest genotoxic potency of all metabolites of 1,3-butadiene. DEB is considered a carcinogen mainly due to its high potential as clastogen, which induces structural chromosome aberrations such as sister chromatid exchanges, chromosomal breaks, and micronuclei. Due to its clastogenic effect, DEB is one of the most used agents for diagnostic studies of Fanconi anemia, a recessively inherited disease related to mutations affecting several genes involved in a common DNA repair pathway. When performing Fanconi anemia diagnostic tests in our laboratory, we have observed occasional multipolar mitosis (MM) in lymphocyte cultures exposed to 0.1 μg/ml of DEB and harvested in the absence of any mitotic spindle inhibitor. Although previous studies reported an aneugenic effect (i.e. it induces aneuploidy) of DEB, no mechanism was suggested to explain such observations. Therefore, the aim of this study was to investigate whether exposure to 0.1 μg/ml of DEB is significantly associated with the occurrence of MM. We blindly assessed the frequency of MM in lymphocyte cultures from 10 nonsmoking healthy individuals. Two series of 3 cultures were performed from each sample under different conditions: A, without DEB; B, with 0.1 μg/ml of DEB, and C, with 25 μM of mitomycin C as positive control. Cultures exposed to DEB showed higher frequencies of MM (23 of 2,000 cells) than did the unexposed ones (3 of 2,000 cells). |
| Databáze: | OpenAIRE |
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