Electron-microscopic cytochemistry of the catecholaminergic innervation of TRH neurons in the rat hypothalamus
| Autor: | Yasumitsu Nakai, S. Sunayama, A. Sato, Seiji Shioda, Yoshimi Shimoda |
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| Rok vydání: | 1986 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Histology endocrine system diseases Immunocytochemistry Hypothalamus Tritium Cerebral Ventricles Pathology and Forensic Medicine Hydroxydopamines Norepinephrine Internal medicine medicine Animals Thyrotropin-Releasing Hormone Electron microscopic Combined method Injections Intraventricular Neurons Catecholaminergic Chemistry Rats Inbred Strains Cell Biology Molecular medicine Axons Rats Microscopy Electron Endocrinology nervous system Cytochemistry Autoradiography hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Cell and Tissue Research. 245 |
| ISSN: | 1432-0878 0302-766X |
| DOI: | 10.1007/bf00213928 |
| Popis: | The catecholaminergic innervation of thyrotropin-releasing hormone (TRH) neurons was examined by use of a combined method of 5-hydroxydopamine (5-OHDA) uptake or autoradiography after intraventricular injection of 3H-noradrenaline (3H-NA) and immunocytochemistry for TRH in the same tissue sections at the electron-microscopic level. TRH-like immunoreactive nerve cell bodies were distributed abundantly in the parvocellular part of the paraventricular nucleus (PVN), in the suprachiasmatic preoptic nucleus and in the dorsomedial nucleus of the rat hypothalamus. In the PVN, a large number of immunonegative axon terminals were found to make synaptic contact with TRH-like immunoreactive cell bodies and fibers. In the combined autoradiography or 5-OHDA labeling with immunocytochemistry, axon terminals labeled with 3H-NA or 5-OHDA were found to form synaptic contacts with the TRH immunoreactive nerve cell bodies and fibers. These findings suggest that catecholamine-containing neurons, probably noradrenergic, may innervate TRH neurons to regulate TRH secretion via synapses with other unknown neurons in the rat PVN. |
| Databáze: | OpenAIRE |
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