Hsa_circ_0001361 facilitates the progress of lung adenocarcinoma cells via targeting miR-525-5p/VMA21 axis
Autor: | Liu-Xi Shi, Le-Ping Fang, Bo-Qiang Fan, Hongyu Shen, Weifei Fan, Lin Wang, Wei Xu, Juqing Xu |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
LUAD
Proliferation medicine.disease_cause General Biochemistry Genetics and Molecular Biology VMA21 Metastasis Nude mouse In vivo medicine Lung cancer biology business.industry Cell growth Research miR-525-5p General Medicine medicine.disease biology.organism_classification Blot circ_0001361 Cancer research Adenocarcinoma Medicine business Carcinogenesis |
Zdroj: | Journal of Translational Medicine, Vol 19, Iss 1, Pp 1-15 (2021) Journal of Translational Medicine |
ISSN: | 1479-5876 |
Popis: | Background Lung adenocarcinoma (LUAD) is a common subtype of lung cancer with high recurrence rate and fatality. Circ_0001361 has been recognized as key regulators in various malignancies, but its roles in LUAD remain ambiguous. Methods Circ_0001361, miR-525-5p, and VMA21 levels were assessed by RT-qPCR. The growth and metastasis of LUAD cells were detected by MTT, colony formation, wound scratch, and transwell assays, respectively. The interaction between circ_0001361/VMA21 and miR-525-5p was detected by dual luciferase, RNA immunoprecipitation, and RNA pull-down assays. VMA21 protein level was detected by Western blotting. Nude mouse xenograft model was established to determine the role of circ_0001361 in tumor growth in vivo. Results Circ_0001361 was up-regulated, while miR-525-5p was down-regulated in LUAD tissues and cells. Functional experiments demonstrated that circ_0001361 drove LUAD cell growth and metastasis. Mechanistically, circ_0001361 functioned as a sponge of miR-525-5p to up-regulate downstream target VMA21 level. MiR-525-5p/VMA21 axis was involved in circ_0001361-mediated malignant phenotypes of LUAD cells. Finally, inhibition of circ_0001361 restrained in vivo xenograft tumor growth via regulating miR-525-5p/VMA21 axis. Conclusion Our findings elucidate that circ_0001361 facilitates the tumorigenesis and development of LUAD through miR-525-5p/VMA21 axis, providing evidence for circ_0001361 as a potential prognosis biomarker and therapeutic target for clinical treatment of LUAD. |
Databáze: | OpenAIRE |
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