MicroRNA expression in relation with clinical evolution of osteosarcoma
| Autor: | Eric G. Ramírez-Salazar, Margarita Valdés-Flores, Linares-González Lm, Alberto Hidalgo-Bravo, Eréndira Estrada-Villaseñor, Rafael Velázquez-Cruz, Lucero Monterde-Cruz, Ernesto Andrés Delgado-Cedillo, Roberto Guzmán-González, Genaro Rico-Martínez |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine Oncology Malignant bone tumor medicine.medical_specialty Adolescent Bone Neoplasms Pathology and Forensic Medicine Metastasis Pathogenesis Young Adult 03 medical and health sciences 0302 clinical medicine Internal medicine microRNA Expression analysis Biomarkers Tumor medicine Humans Neoplasm Invasiveness Child Osteosarcoma business.industry Cell Biology medicine.disease body regions MicroRNAs 030104 developmental biology 030220 oncology & carcinogenesis Potential biomarkers embryonic structures Female business Function (biology) |
| Zdroj: | Pathology - Research and Practice. 216:153038 |
| ISSN: | 0344-0338 |
| DOI: | 10.1016/j.prp.2020.153038 |
| Popis: | Osteosarcoma is the most common malignant bone tumor. Early diagnosis remains a major challenge, mainly because of the lack of specific biomarkers. We performed miRNAs expression analysis through qPCR in affected and paired healthy bone derived from osteosarcoma patients. Hierarchical clustering using the top ten miRNAs with differential expression showed two main clusters. One integrated by patients with the presence of metastasis or relapse and the other without these complications. Further pathway enrichment analysis reduced to four main miRNAs, hsa-miR-486-3p, hsa-miR-355-5p, hsa-miR-34a-5p and hsa-miR-1228-3p. Afterwards, we compared patients with and without metastasis, the function enrichment analysis along with review of relevant literature, showed that hsa-miR-93-5p and hsa-miR-28-5p were associated with metastasis development. Our results support the relevance of miRNAs in the pathogenesis of osteosarcoma and contribute with evidence regarding the potential role of miRNAs as potential biomarkers. More studies are needed to define the most informative miRNAs in osteosarcoma patients. |
| Databáze: | OpenAIRE |
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