Rat Liver Cytosolic Retinal Dehydrogenase: Comparison of 13-cis-, 9-cis-, and all-trans-Retinal as Substrates and Effects of Cellular Retinoid-Binding Proteins and Retinoic Acid on Activity
| Autor: | Joseph L. Napoli, Zeyad El Akawi |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
Retinal dehydrogenase Retinoic acid Tretinoin Dehydrogenase Biology Binding Competitive Biochemistry Substrate Specificity Rats Sprague-Dawley chemistry.chemical_compound Cytosol Allosteric Regulation medicine Animals Isoelectric Point chemistry.chemical_classification Binding protein Retinoid binding protein Retinal Dehydrogenase Retinol-Binding Proteins Cellular Stereoisomerism Retinal Aldehyde Oxidoreductases Rats Retinol-Binding Proteins Kinetics Enzyme Liver chemistry Retinaldehyde medicine.drug |
| Zdroj: | Biochemistry. 33:1938-1943 |
| ISSN: | 1520-4995 0006-2960 |
| Popis: | A basic pI retinal dehydrogenase has been purified recently that accounts for approximately 90% of the all-trans-retinal dehydrogenase activity of rat liver cytosol. In this work, we show that this enzyme also accounts for approximately 90% of the 9-cis-retinal dehydrogenase activity of rat liver cytosol. The partially-purified enzyme displayed allosteric kinetics for 9-cis-retinal [K0.5 = 5.2 microM, Hill coefficient = 1.4, Vmax = 7.85 nmol min-1 (mg of protein)-1] with the ratio Vmax/K0.5 = 1.5. The latter is similar to that of 2.1 for all-trans-retinal [K0.5 = 1.6 microM, Hill coefficient = 1.4, Vmax = 3.4 nmol min-1 (mg of protein)-1]. Competition between all-trans- and 9-cis-retinal occurred only when micromolar concentrations of both were present, indicating that the dehydrogenase could catalyze both all-trans- and 9-cis-retinoic acid syntheses simultaneously at the nanomolar amounts of the retinals that are likely to occur physiologically. Although reactions of all-trans- and 9-cis-retinoids were catalyzed with similar efficiencies, 13-cis-retinal was not an efficient substrate. This retinal dehydrogenase was not feedback-inhibited by all-trans- or 9-cis-retinoic acid, nor by holocellular retinoic acid-binding protein, but was stimulated modestly by apocellular retinoic acid-binding protein, an effect not observed in the presence of cellular retinol-binding protein. These data indicate that products, via feedback inhibition, do not regulate retinoic acid synthesis by this dehydrogenase. This dehydrogenase may serve as a common enzyme in the conversion of all-trans- and 9-cis-retinal into their acids. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|