Characterization of the mRNA untranslated regions [UTR] of the Trypanosoma cruzi LYT1 isoforms derived by alternative trans-splicing
| Autor: | Julián Camilo Casas, Elizabeth Ruiz, Concepción J. Puerta, César A. Ramírez, María Isabel Ospina, Jose M. Requena |
|---|---|
| Přispěvatelé: | Colciencias (Colombia) |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Gene isoform Untranslated region Untranslatedregion [UTR] Polyadenylation Untranslated region [UTR] Trypanosoma cruzi 030231 tropical medicine Trans-splicing RNA-binding protein Computational biology LYT1 gene Biology 03 medical and health sciences 0302 clinical medicine Trypanosomacruzi Gene expression RNAbinding proteins [RBP] Regulation of gene expression lcsh:Science (General) Gene Multidisciplinary Regulationofgeneexpression RNA binding proteins [RBP] 030104 developmental biology lcsh:Q1-390 |
| Zdroj: | Universitas Scientiarum, Vol 23, Iss 2, Pp 267-290 (2018) Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 2027-1352 0122-7483 |
| Popis: | In trypanosomatids,geneexpressionismainlyregulated at posttranscriptional level,throughmechanisms based on the interaction between RNA Binding Proteins [RBPs] and motifs present in the untranslated regions [UTRs] of Them RNAs,whichaltogetherformribonucleoproteiccomplexes [RNP] that define thefateofthemRNA.Thepre-mRNAderivedfromthe LYT1 gene of Trypanosomacruzi, isprocessedbyalternative trans-splicing, resultingin differentmRNAswhichcodefortheisoformsmLYT1andkLYT1,proteins having differential expression,cellular location and function.The aim of this study was to characterizethe5¿and3¿UTRsofthe LYT1 mRNAsasthe initial steptowardstheobjectiveofidentificationoftheRBPsresponsiblefor theirdifferentialexpression.Thepresenceofthetwotypesof5¿UTRswere confirmedintwo T.cruzi isolatesbelongingtotheDTUI,thus,corroborating theoccurrenceofalternative trans-splicing alsointhe LYT1 geneofthis T.cruzi DTU.Inaddition,forthefirsttime,wasunscoveredtheexistenceoftwo types of LYT1 mRNAstranscripts,differinginlengthby116nts,thatare generatedbyalternativepolyadenylation.Furthermore,an in-silico analysis of theexperimentallyobtainedUTRs,andtenadditional LYT1 sequences retrievedfromTritrypDBandGenBankdatabases,togetherwithathoroughly searchofstructuralmotifs,showedaremarkableconservationofrelevant structuralmotifspreviouslyassociatedwithRNAmetabolisminthedifferent UTRs; theseelementsmightbeinvolvedinthedifferentialstage COLCIENCIAS research project ID PPTA 120356933228 |
| Databáze: | OpenAIRE |
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