Alteration of bacterial communities and organic matter in microbial fuel cells (MFCs) supplied with soil and organic fertilizer

Autor: Stefano Mocali, Elena Di Mattia, Anna Benedetti, Giovanna Bianconi, Francesco Canganella, Elena Perrin, Maria Teresa Dell'Abate, Renato Fani, Carlo Galeffi, Alessandro Florio, Melania Migliore
Přispěvatelé: Research Centre for Agrobiology and Pedology (CRA-ABP), Research Centre for the Soil-Plant System, Evolutionary Biology Department, Universtiy of Florence, Department for innovation in biological, agro-food and forest systems (DIBAF), University of Tuscia, Tuscia University, Italian Ministry of Agricultural, Food, and Forestry Policies (MIPAAF), BEM project D.M. 247/07
Jazyk: angličtina
Rok vydání: 2013
Předmět:
DNA
Bacterial
Microbial fuel cell
Bioelectric Energy Sources
[SDV]Life Sciences [q-bio]
Molecular Sequence Data
010501 environmental sciences
Biology
DNA
Ribosomal
01 natural sciences
Applied Microbiology and Biotechnology
Actinobacteria
soil
03 medical and health sciences
microbial fuel cell
Electricity
RNA
Ribosomal
16S
Botany
Cluster Analysis
Organic matter
Food science
humification
Organic Chemicals
Fertilizers
030304 developmental biology
0105 earth and related environmental sciences
organic matter
chemistry.chemical_classification
0303 health sciences
electrogenic bacteria
Bacteria
Denaturing Gradient Gel Electrophoresis
Soil organic matter
Genetic Variation
Sequence Analysis
DNA
General Medicine
Mineralization (soil science)
biology.organism_classification
Biota
Humus
Random Amplified Polymorphic DNA Technique
chemistry
microbial diversity
Organic fertilizer
Temperature gradient gel electrophoresis
Biotechnology
Zdroj: Applied Microbiology and Biotechnology
Applied Microbiology and Biotechnology, Springer Verlag, 2013, 97 (3), pp.1299-1315. ⟨10.1007/s00253-012-3906-6⟩
ISSN: 0175-7598
1432-0614
DOI: 10.1007/s00253-012-3906-6⟩
Popis: International audience; The alteration of the organic matter (OM) and the composition of bacterial community in microbial fuel cells (MFCs) supplied with soil (S) and a composted organic fertilizer (A) was examined at the beginning and at the end of 3 weeks of incubation under current-producing as well as no-current-producing conditions. Denaturing gradient gel electrophoresis revealed a significant alteration of the microbial community structure in MFCs generating electricity as compared with no-current-producing MFCs. The genetic diversity of cultivable bacterial communities was assessed by random amplified polymorphic DNA (RAPD) analysis of 106 bacterial isolates obtained by using both generic and elective media. Sequencing of the 16S rRNA genes of the more representative RAPD groups indicated that over 50.4% of the isolates from MFCs fed with S were Proteobacteria, 25.1% Firmicutes, and 24.5% Actinobacteria, whereas in MFCs supplied with A 100% of the dominant species belonged to gamma-Proteobacteria. The chemical analysis performed by fractioning the OM and using thermal analysis showed that the amount of total organic carbon contained in the soluble phase of the electrochemically active chambers significantly decreased as compared to the no-current-producing systems, whereas the OM of the solid phase became more humified and aromatic along with electricity generation, suggesting a significant stimulation of a humification process of the OM. These findings demonstrated that electroactive bacteria are commonly present in aerobic organic substrates such as soil or a fertilizer and that MFCs could represent a powerful tool for exploring the mineralization and humification processes of the soil OM.
Databáze: OpenAIRE
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