Silencing miR-106b accelerates osteogenesis of mesenchymal stem cells and rescues against glucocorticoid-induced osteoporosis by targeting BMP2
| Autor: | Ke Zen, Ke Liu, Chen-Yu Zhang, Ying Jing, Donghai Li, Huan Zhao, Xichao Zhou, Xuejie Fu, Wen Zhang, Huilin Yang, Yan Zhang, Qin Shi, Yunxia Tao |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Histology Physiology Placenta Endocrinology Diabetes and Metabolism Osteoporosis Regulator Bone Morphogenetic Protein 2 Smad Proteins Biology Bone morphogenetic protein 2 Dexamethasone Bone resorption 03 medical and health sciences 0302 clinical medicine Osteogenesis Pregnancy Internal medicine microRNA Gene expression medicine Animals Humans Gene silencing Gene Silencing Bone Resorption Glucocorticoids Osteoblasts Base Sequence Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells medicine.disease Mice Inbred C57BL MicroRNAs 030104 developmental biology Endocrinology Gene Knockdown Techniques 030220 oncology & carcinogenesis Cancer research Female |
| Zdroj: | Bone. 97:130-138 |
| ISSN: | 8756-3282 |
| DOI: | 10.1016/j.bone.2017.01.014 |
| Popis: | Osteoporosis is a serious health problem worldwide. MicroRNA is a post-transcriptional regulator of gene expression by either promoting mRNA degradation or interfering with mRNA translation of specific target genes. It plays a significant role in the pathogenesis of osteoporosis. Here, we first demonstrated that miR-106b (miR-106b-5p) negatively regulated osteogenic differentiation of mesenchymal stem cells in vitro. Then, we found that miR-106b expression increased in C57BL/6 mice with glucocorticoid-induced osteoporosis (GIOP), and that silencing of miR-106b signaling protected mice against GIOP through promoting bone formation and inhibiting bone resorption. At last, we showed that miR-106b inhibited osteoblastic differentiation and bone formation partly through directly targeting bone morphogenetic protein 2 (BMP2) both in vitro and in the GIOP model. Together, our findings have identified the role and mechanism of miR-106b in negatively regulating osteogenesis. Inhibition of miR-106b might be a potential new strategy for treating osteoporosis and bone defects. |
| Databáze: | OpenAIRE |
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