The fate of biodegradable microspheres injected into rat brain
| Autor: | Carey McInnis, David W. Mason, William W. Snow, Thomas R. Tice, Darryl F. Love, Jay K. Staas, Anthony P. Nicholas, Kiran Gupta, Teresa M. Ferrell |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Pathology medicine.medical_specialty Polymers Phagocytosis Biocompatible Materials Rats Sprague-Dawley Polylactic Acid-Polyglycolic Acid Copolymer In vivo medicine Animals Lactic Acid Injections Intraventricular Glial fibrillary acidic protein biology Microglia Chemistry General Neuroscience Brain In vitro Microspheres Rats medicine.anatomical_structure Astrocytes Drug delivery Biophysics biology.protein Neuroglia Polyglycolic Acid Astrocyte |
| Zdroj: | Neuroscience letters. 323(2) |
| ISSN: | 0304-3940 |
| Popis: | Biodegradable microspheres made with poly-[D,L-lactide-co-glycolide] represent an evolving technology for drug delivery into the central nervous system. Even though these microspheres have been shown to be engulfed by astrocytes in vitro, the purpose of the present study was to track the fate of biodegradable microspheres in vivo. This was accomplished using microspheres containing the fluorescent dye coumarin-6 followed 1 day, 1 week and 1 month after intracerebral injections of this material were made into the rat brain. Using dual color immunohistochemistry and antisera against glial fibrillary acidic protein for astrocytes versus phosphotyrosine for microglia, results demonstrate that phagocytosis of small coumarin-containing microspheres7.5 microm in diameter was primarily by microglia in vivo during the first week post-injection. In contrast, only a small minority of these microspheres appeared to be engulfed by astrocytes. |
| Databáze: | OpenAIRE |
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