Neural differentiation medium for human pluripotent stem cells to model physiological glucose levels in human brain
| Autor: | Mary Familari, Serena Viventi, Robb U. de Iongh, Alexandra J Harvey, Mitchell St Clair-Glover, Michal E. Mor, Mirella Dottori, Fergus J. Cameron |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Brain development General Neuroscience Insulin medicine.medical_treatment Induced Pluripotent Stem Cells Brain Cell Differentiation Human brain Biology Cell biology Culture Media 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Glucose Neurosphere medicine Humans Neural differentiation Induced pluripotent stem cell Signalling pathways Neural development 030217 neurology & neurosurgery |
| Zdroj: | Brain research bulletin. 173 |
| ISSN: | 1873-2747 |
| Popis: | Cortical neurospheres (NSPs) derived from human pluripotent stem cells (hPSC), have proven to be a successful platform to investigate human brain development and neuro-related diseases. Currently, many of the standard hPSC neural differentiation media, use concentrations of glucose (approximately 17.5-25 mM) and insulin (approximately 3.2 μM) that are much greater than the physiological concentrations found in the human brain. These culture conditions make it difficult to analyse perturbations of glucose or insulin on neuronal development and differentiation. We established a new hPSC neural differentiation medium that incorporated physiological brain concentrations of glucose (2.5 mM) and significantly reduced insulin levels (0.86 μM). This medium supported hPSC neural induction and formation of cortical NSPs. The revised hPSC neural differentiation medium, may provide an improved platform to model brain development and to investigate neural differentiation signalling pathways impacted by abnormal glucose and insulin levels. |
| Databáze: | OpenAIRE |
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