Cross-tissue translational capacities I. The adequacy of tRNAs from heterologous tissues in the translation of hemoglobin message
| Autor: | Gerald P. Hirsch, Bernard L. Strehler |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Aging
Reticulocytes Acylation Cellular differentiation Heterologous Biology Kidney Amino Acyl-tRNA Synthetases Hemoglobins RNA Transfer Reticulocyte In vivo Protein biosynthesis medicine Animals Brain Valine Translation (biology) medicine.anatomical_structure Liver Biochemistry Genetic Code Protein Biosynthesis Transfer RNA Rabbits Hemoglobin Developmental Biology |
| Zdroj: | Mechanisms of Ageing and Development. 2:229-235 |
| ISSN: | 0047-6374 |
| DOI: | 10.1016/0047-6374(73)90019-5 |
| Popis: | Experiments designed to test the theory that translational modulation is a major agent in the control of development have been conducted. These experiments employed a cell-free hemoglobin synthesizing system derived from rabbit reticulocytes. Comparison of protein synthesis rates observed when non-mitochondrial tRNAs derived from brain, kidney, liver or reticulocytes were employed indicates that heterologous tissue tRNA complements are quite capable of supporting a nearly maximum rate of protein synthesis. Heterologous tRNAs translate reticulocyte messages more slowly than do reticulocyte tRNAs at non-saturating concentrations of equivalent acceptor activity. The results appear to indicate that hemoglobin production is not limited in vivo by the translational capacities of tRNAs present in these specific differentiated cell types. |
| Databáze: | OpenAIRE |
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