Liquid chromatographic determination of ampicillin in bovine and dog plasma by using a tandem solid-phase extraction method
| Autor: | J Vandenbranden, Hans Nelis, A. de Kruif, A.P. De Leenheer, B. J. Verhaeghe, D Mattheeuws |
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| Rok vydání: | 1992 |
| Předmět: |
Pharmacology
Detection limit Cephalexin Chromatography Chemistry Extraction (chemistry) Capsules High-performance liquid chromatography Injections Intramuscular Dosage form Infectious Diseases Column chromatography Dogs Animals Pharmacology (medical) Ampicillin Cattle Spectrophotometry Ultraviolet Solid phase extraction Selectivity Chromatography High Pressure Liquid Antibacterial agent Tablets Research Article |
| Zdroj: | Antimicrobial agents and chemotherapy. 36(8) |
| ISSN: | 0066-4804 |
| Popis: | The determination of ampicillin in plasma and serum by reversed-phase high-performance liquid chromatography with UV detection suffers from poor selectivity and sensitivity. Currently, the most common approach to overcoming these problems consists of improving the compound's detectability via pre- or postcolumn derivatization. In the method that we describe, however, enhanced selectivity is afforded by sample purification by a tandem solid-phase extraction method (ion-exchange and reversed-phase). This approach permits detection at wavelengths of as low as 210 nm, which results in enhanced sensitivity (detection limit, 0.01 microgram/ml). A second factor that affects selectivity is the addition to the chromatographic eluent of a crown ether to optimize the separation between ampicillin and polar endogenous plasma constituents. This combination of improved sample pretreatment and a more selective chromatographic system in conjunction with internal standardization forms the basis of a new assay for the quantitation of ampicillin in plasma. The overall recovery of ampicillin was 76.4% +/- 4.9% (n = 24), and the within-run and between-run coefficients of variation ranged from 1.6 to 7.2%. The method was applied to pharmacokinetic studies in cows and dogs after intramuscular or oral administration of the drug. |
| Databáze: | OpenAIRE |
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