Alterations in Ca2+-binding on plasma membrane after adaptation to salt stress of tobacco cells in suspension
Autor: | Yoshiyuki Murata, Ichiro Obi, Shinichi Katsura, Tadaaki Kakutani |
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Rok vydání: | 2000 |
Předmět: |
Physiology
Nicotiana tabacum Potassium chemistry.chemical_element Salt (chemistry) Plant Science Calcium Sodium Chloride Binding Competitive Membrane Potentials Tobacco Surface charge chemistry.chemical_classification biology Dose-Response Relationship Drug Cell Membrane Sodium Cell Biology General Medicine Protoplast Cations Monovalent biology.organism_classification Adaptation Physiological Electrophoresis Plants Toxic Membrane chemistry Biochemistry Biophysics |
Zdroj: | Plantcell physiology. 41(11) |
ISSN: | 0032-0781 |
Popis: | Electrophoresis was used to study effects of salinity on the characteristics of Ca2+ binding to the outer surface of plasma membrane (PM) of protoplasts isolated from two types of tobacco (Nicotiana tabacum L., cv. Bright Yellow) cultured cells that were adapted (tolerant) and unadapted (sensitive) to 50 mM NaCl stress. Electrophoretic analysis of salt-sensitive NaCl-unadapted cells shows that Na+ induced an appreciably higher degree of reduction in the amount of Ca2+ bound to PM compared with K+ with increasing concentration from 0.1 to 30 mM. In salt-tolerant NaCl-adapted cells, however, both Na+ and K+ ions induced almost the same degree of reduction in the amount of Ca2+ bound to PM in the physiological concentration range of Ca2+ in the medium between 2 and 4 mM. These results suggest that, under the physiological conditions, PM of salt-sensitive NaCl-unadapted cells has an appreciable amount of PM-bound Ca2+ that is desorbed much easier by Na+ than K+, whereas PM of salt-tolerant NaCl-adapted cells has the PM-bound Ca2+ that can be equally desorbed by Na+ and K+. |
Databáze: | OpenAIRE |
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