Proliferative potential and phenotypic analysis of long-term cultivated human granulosa cells initiated by addition of follicular fluid
| Autor: | Lenka Bruckova, Tomáš Soukup, Eva Brcakova, Lenka Kucerova, Stanislav Micuda, Jaroslav Mokry, Jana Pavelkova, Jiri Moos, Benjamin Visek, Karel Rezabek, Martina Moosova |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
endocrine system
medicine.medical_specialty Time Factors Cell Culture Techniques Reproductive medicine Biology Technical Innovations Phenotypic analysis Genetics medicine Humans Cells Cultured Genetics (clinical) Cell Proliferation Granulosa Cells food and beverages Obstetrics and Gynecology General Medicine Telomere Flow Cytometry Phenotype Follicular fluid eye diseases Human genetics Follicular Fluid Cell biology Reproductive Medicine Karyotyping Female sense organs hormones hormone substitutes and hormone antagonists Developmental Biology |
| Zdroj: | Journal of Assisted Reproduction and Genetics. 28:939-950 |
| ISSN: | 1573-7330 1058-0468 |
| DOI: | 10.1007/s10815-011-9617-6 |
| Popis: | The aim of this study was to develop and optimize a strategy for long-term cultivation of luteinizing human granulosa cells (GCs).GCs were cultivated in DMEM/F12 medium supplemented with 2% fetal calf serum. In vitro proliferation of GCs was supported by follicular fluid as well as FSH and growth factors.The cultured GCs were maintained for 45 days with a doubling time of 159 ± 24 h. GCs initiated by the addition of follicular fluid and cultivated under low serum conditions reached 10 ± 0.7 population doublings. GCs maintain the typical phenotypic expression and the telomere length according to specific culture conditions.Our present study has demonstrated that GCs can be maintained in vitro for at least 45 days and this cell model can be beneficial when studying hormonal regulation associated with follicular maturation and preparation of oocytes for fertilization. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink