Epigenetic control of myeloid cells behavior by Histone Deacetylase activity (HDAC) during tissue and organ regeneration in Xenopus laevis
| Autor: | Fernanda Martins de Almeida, Michael Levin, Fellipe Soares dos Santos Cardoso, Thayse Pinheiro da Costa, Nathalia Pentagna, José G. Abreu, Katia Carneiro, Ana Maria Blanco Martinez |
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| Rok vydání: | 2020 |
| Předmět: |
Myeloid
Organogenesis Immunology Xenopus Regenerative Medicine Regenerative medicine Histone Deacetylases Epigenesis Genetic 03 medical and health sciences Xenopus laevis 0302 clinical medicine Lipid droplet medicine Animals Humans Regeneration Myeloid Cells Epigenetics Cells Cultured 030304 developmental biology 0303 health sciences biology Regeneration (biology) Gene Expression Regulation Developmental Cell Differentiation biology.organism_classification Cell biology medicine.anatomical_structure Histone deacetylase activity Histone deacetylase 030217 neurology & neurosurgery Biomarkers Developmental Biology |
| Zdroj: | Developmental and comparative immunology. 114 |
| ISSN: | 1879-0089 |
| Popis: | In the present work we have focused on the Histone Deacetylase (HDAC) control of myeloid cells behavior during Xenopus tail regeneration. Here we show that myeloid differentiation is crucial to modulate the regenerative ability of Xenopus tadpoles in a HDAC activity-dependent fashion. HDAC activity inhibition during the first wave of myeloid differentiation disrupted myeloid cells dynamics in the regenerative bud as well the mRNA expression pattern of myeloid markers, such as LURP, MPOX, Spib and mmp7. We also functionally bridge the spatial and temporal dynamics of lipid droplets, the main platform of lipid mediators synthesis in myeloid cells during the inflammatory response, and the regenerative ability of Xenopus tadpoles. In addition, we showed that 15-LOX activity is necessary during tail regeneration. Taken together our results support a role for the epigenetic control of myeloid behavior during tissue and organ regeneration, which may positively impact translational approaches for regenerative medicine. |
| Databáze: | OpenAIRE |
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